Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness

被引：100

作者：

Han, SW

Nakamura, C

Obataya, I

Nakamura, N

Miyake, J

机构：

[1] AIST, RICE, Amagasaki, Hyogo 6610974, Japan

[2] Tokyo Univ Agr & Technol, Div Biotechnol & Life Sci, Koganei, Tokyo 1848588, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 332卷 / 03期

关键词：

single cell manipulation; nanoneedle; invasiveness; cell surgery gene expression;

D O I：

10.1016/j.bbrc.2005.04.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously demonstrated a new cell manipulation technology by using an atomic force microscope (AFM) and ultrathin needles, named nanoneedles. The nanoneedle is an AFM tip etched by a focused ion beam (FIB) and is sharpened from 200 to 800 nm in diameter. In this study, we have evaluated the proper diameter of a needle required for insertion into human cells over a long period without causing cell death, and achieved highly efficient gene expression method for human cells using a nanoneedle and an AFM. (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：633 / 639

页数：7

相关论文

共 16 条

[1] Thimerosal induces DNA breaks, caspase-3 activation, membrane damage, and cell death in cultured human neurons and fibroblasts [J].

Baskin, DS ;

Ngo, H ;

Didenko, VV .

TOXICOLOGICAL SCIENCES, 2003, 74 (02) :361-368

[2] VIABILITY OF CRYPTOSPORDIUM-PARVUM OOCYSTS - CORRELATION OF INVITRO EXCYSTATION WITH INCLUSION OR EXCLUSION OF FLUOROGENIC VITAL DYES [J].

CAMPBELL, AT ;

ROBERTSON, LJ ;

SMITH, HV .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (11) :3488-3493

[3] The capsaicin receptor: a heat-activated ion channel in the pain pathway [J].

Caterina, MJ ;

Schumacher, MA ;

Tominaga, M ;

Rosen, TA ;

Levine, JD ;

Julius, D .

NATURE, 1997, 389 (6653) :816-824

[4] Mitochondria and apoptosis [J].

Green, DR ;

Reed, JC .

SCIENCE, 1998, 281 (5381) :1309-1312

[5] A molecular delivery system by using AFM and nanoneedle [J].

Han, SW ;

Nakamura, C ;

Obataya, I ;

Nakamura, N ;

Miyake, J .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (10) :2120-2125

[6] A comparison of the activity, sequence specificity, and CRM1-dependence of different nuclear export signals [J].

Henderson, BR ;

Eleftheriou, A .

EXPERIMENTAL CELL RESEARCH, 2000, 256 (01) :213-224

[7] A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes [J].

Knoblauch, M ;

Hibberd, JM ;

Gray, JC ;

van Bel, AJE .

NATURE BIOTECHNOLOGY, 1999, 17 (09) :906-909

[8] Sieve tubes in action [J].

Knoblauch, M ;

van Bel, AJE .

PLANT CELL, 1998, 10 (01) :35-50

[9] Lipid-assisted microinjection: Introducing material into the cytosol and membranes of small cells [J].

Laffafian, I ;

Hallett, MB .

BIOPHYSICAL JOURNAL, 1998, 75 (05) :2558-2563

[10] Determinants of the nuclear localization of the heterodimeric DNA fragmentation factor (ICAD/CAD) [J].

Lechardeur, D ;

Drzymala, L ;

Sharma, M ;

Zylka, D ;

Kinach, R ;

Pacia, J ;

Hicks, C ;

Usmani, N ;

Rommens, JM ;

Lukacs, GL .

JOURNAL OF CELL BIOLOGY, 2000, 150 (02) :321-334