Over-expression of GATA-6 in Xenopus embryos blocks differentiation of heart precursors

被引:95
作者
Gove, C
Walmsley, M
Nijjar, S
Bertwistle, D
Guille, M
Partington, G
Bomford, A
Patient, R
机构
[1] UNIV LONDON KINGS COLL, RANDALL INST, DIV BIOMED SCI, DEV BIOL RES CTR, LONDON WC2B 5RL, ENGLAND
[2] UNIV LONDON KINGS COLL, SCH MED & DENT, INST LIVER STUDIES, MAURICE WOHL LAB, LONDON SE5 9PJ, ENGLAND
基金
英国惠康基金;
关键词
cardiac development; differentiation; GATA transcription factors; RNA injection; Xenopus;
D O I
10.1093/emboj/16.2.355
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Xenopus GATA-6 transcripts are first detected at the beginning of gastrulation in the mesoderm, and subsequent domains of expression include the field of cells shown to have heart-forming potential, In this region, GATA-6 expression continues only in those cells that go on to form the heart; however, a decrease occurs prior to terminal differentiation. Artificial elevation of GATA-6, but not GATA-1, prevents expression of both cardiac actin and heart-specific myosin light chain, This effect is heart-specific because cardiac actin expression is unaffected in somites, Expression of the earlier marker XNkx-2.5 was unaffected and morphological development of the heart was initiated independently of the establishment of the contractile machinery, We conclude that a reduction in the level of GATA-6 is important for the progression of the cardiomyogenic differentiation programme and that GATA-6 may act to maintain heart cells in the precursor state, At later stages, when the elevated GATA-6 levels had decayed, differentiation ensued but the number of cells contributing to the myocardium had increased, suggesting either that the blocked cells had proliferated or that additional cells had been recruited.
引用
收藏
页码:355 / 368
页数:14
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