Partial Excision of blaKPC from Tn4401 in Carbapenem-Resistant Klebsiella pneumoniae

被引：33

作者：

Chen, Liang ^{[1
]}

Chavda, Kalyan D. ^{[1
]}

Mediavilla, Jose R. ^{[1
]}

Jacobs, Michael R. ^{[2
]}

Levi, Michael H. ^{[3
,4
]}

Bonomo, Robert A. ^{[5
,6
,7
,8
,9
]}

Kreiswirth, Barry N. ^{[1
]}

机构：

[1] Univ Med & Dent New Jersey, Publ Hlth Res Inst TB Ctr, Int Ctr Publ Hlth, Newark, NJ 07103 USA

[2] Univ Hosp Case Med Ctr, Dept Pathol, Cleveland, OH USA

[3] Montefiore Med Ctr, Div Pathol, Bronx, NY 10467 USA

[4] Albert Einstein Coll Med, Bronx, NY 10467 USA

[5] Louis Stokes Cleveland VA Med Ctr, Res Serv, Cleveland, OH USA

[6] Case Western Reserve Univ, Dept Med, Cleveland, OH 44106 USA

[7] Case Western Reserve Univ, Dept Pharmacol, Cleveland, OH 44106 USA

[8] Case Western Reserve Univ, Dept Mol Biol, Cleveland, OH 44106 USA

[9] Case Western Reserve Univ, Dept Microbiol, Cleveland, OH 44106 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 03期

基金：

美国国家卫生研究院;

关键词：

BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS; EXPANSION; PLASMIDS; STRAINS; GENES;

D O I：

10.1128/AAC.06182-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a novel Tn4401 variant (Tn4401d) in epidemic Klebsiella pneumoniae clone ST258, from which a partial bla(KPC) fragment has been excised along with ISKpn7 and a partial tnpA fragment. Nested-PCR experiments confirmed that this region can be removed from distinct Tn4401 isoforms in both K. pneumoniae and Escherichia coli. This study highlights that the region surrounding bla(KPC) is undergoing recombination and that Tn4401 itself is heterogeneous and highly plastic.

引用

页码：1635 / 1638

页数：4

共 20 条

[1] Epidemiological Expansion, Structural Studies, and Clinical Challenges of New β-Lactamases from Gram-Negative Bacteria
Bush, Karen
Fisher, Jed F.
[J]. ANNUAL REVIEW OF MICROBIOLOGY, VOL 65, 2011, 65 : 455 - 478
[2] Identification of plasmids by PCR-based replicon typing
Carattoli, A
Bertini, A
Villa, L
Falbo, V
Hopkins, KL
Threlfall, EJ
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 63 (03) : 219 - 228
[3] Multiplex Real-Time PCR Assay for Detection and Classification of Klebsiella pneumoniae Carbapenemase Gene (blaKPC) Variants
Chen, Liang
Mediavilla, Jose R.
Endimiani, Andrea
Rosenthal, Marnie E.
Zhao, Yanan
Bonomo, Robert A.
Kreiswirth, Barry N.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (02) : 579 - 585
[4] Clinical and Laboratory Standards Institute (CLSI), 2010, M100S20U CLSI
[5] Functional Characterization of Tn4401, a Tn3-Based Transposon Involved in blaKPC Gene Mobilization
Cuzon, Gaelle
Naas, Thierry
Nordmann, Patrice
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (11) : 5370 - 5373
[6] Wide Dissemination of Pseudomonas aeruginosa Producing β-Lactamase blaKPC-2 Gene in Colombia
Cuzon, Gaelle
Naas, Thierry
Villegas, Maria-Virginia
Correa, Adriana
Quinn, John P.
Nordmann, Patrice
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (11) : 5350 - 5353
[7] Worldwide Diversity of Klebsiella pneumoniae That Produces β-Lactamase blaKPC-2 Gene
Cuzon, Gaelle
Naas, Thierry
Truong, HaVy
Villegas, Maria-Virginia
Wisell, Karin T.
Carmeli, Yehuda
Gales, Ana C.
Navon-Venezia, Shiri
Quinn, John P.
Nordmann, Patrice
[J]. EMERGING INFECTIOUS DISEASES, 2010, 16 (09) : 1349 - 1356
[8] Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae
Dallenne, Caroline
Da Costa, Anaelle
Decre, Dominique
Favier, Christine
Arlet, Guillaume
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (03) : 490 - 495
[9] Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates
Diancourt, L
Passet, V
Verhoef, J
Grimont, PAD
Brisse, S
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) : 4178 - 4182
[10] Genetic Organization of Transposase Regions Surrounding blaKPC Carbapenemase Genes on Plasmids from Klebsiella Strains Isolated in a New York City Hospital
Gootz, Thomas D.
Lescoe, Mary Kay
Dib-Hajj, Fadia
Dougherty, Brian A.
He, Wen
Della-Latta, Phyllis
Huard, Richard C.
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (05) : 1998 - 2004

← 1 2 →