Gold nanorod bioconjugates

被引：374

作者：

Liao, HW

Hafner, JH

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77005 USA

[2] Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA

来源：

CHEMISTRY OF MATERIALS | 2005年 / 17卷 / 18期

关键词：

D O I：

10.1021/cm050935k

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Gold nanorods have been stabilized, conjugated to antibodies, and characterized for biological applications. The stabilizing surfactant bilayer which surrounds gold nanorods was replaced by thiol terminated methoxypoly (ethylene glycol) so that the nanorods are stable in buffer solutions free of surfactant. Nanorod bioconjugation was accomplished with a heterobifunctional cross-linker, with antibody activity confirmed by a strip plate assay. Nanorod bioconjugates were characterized by independent measurements of the nanorod and antibody concentrations. The nanorod molar extinction coefficient was measured from dense yet well separated nanorod films to be 4.4 +/- 0.5 x 10(9) M cm(-1), thus allowing quantitative determination of nanorod concentrations. However, a colorimetric protein assay overestimated the antibody concentration and is therefore likely perturbed by the presence of the nanorods electromagnetically and/or chemically.

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页码：4636 / 4641

页数：6

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