Can redox-sensitive fluorescent probes measure intracellular redox potentials?

被引：2

作者：

Maulucci, G. ^{[1
]}

Pani, G. ^{[2
]}

Papi, M. ^{[1
]}

Galeotti, T. ^{[2
]}

Arcovito, G. ^{[1
]}

De Spirito, M. ^{[1
]}

机构：

[1] Univ Cattolica Sacro Cuore, Ist Fis, Rome, Italy

[2] Univ Cattolica Sacro Cuore, Ist Patol Gen, Rome, Italy

来源：

NUOVO CIMENTO DELLA SOCIETA ITALIANA DI FISICA B-BASIC TOPICS IN PHYSICS | 2010年 / 125卷 / 5-6期

关键词：

DISULFIDE BOND FORMATION; CELL;

D O I：

10.1393/ncb/i2010-10865-7

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

The generation of ratiometric, glutathione specific, redox-sensitive variants of fluorescent probes enable real-time monitoring of intracellular redox changes by making it possible to cancel out most or all of the possible variability caused by instrument efficiency and effective dye content. The probes permit an indirect measure of the glutathione intracellular redox potential E-G, achieved by using the Nernst equation after the measurement of intracellular glutathione redox state. However, E-G is strongly dependent on pH and on the total concentration of glutathione, and since their intracellular spatial distribution is not isotropic, the measurement of E-G may be affected by systematic errors. Here, we show that these errors are not negligible and we suggest possible methods to cancel them out when measuring the redox state of the glutathione redox couple.

引用

页码：645 / 655

页数：11

共 20 条

[11] A NOVEL FLUORESCENCE RATIOMETRIC METHOD CONFIRMS THE LOW SOLVENT VISCOSITY OF THE CYTOPLASM [J].