A CdtA-CdtC complex can block killing of HeLa cells by Haemophilus ducreyi cytolethal distending toxin

被引：31

作者：

Deng, KP ^{[1
]}

Hansen, EJ ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Microbiol, Dallas, TX 75390 USA

来源：

INFECTION AND IMMUNITY | 2003年 / 71卷 / 11期

关键词：

D O I：

10.1128/IAI.71.11.6633-6640.2003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The cytolethal distending toxin (CDT) of Haemophilus ducreyi is comprised of the CdtA, CdtB, and CdtC proteins, with the CdtB protein having demonstrated enzymatic (i.e., DNase) activity. Using a single recombinant Escherichia coli strain with two plasmids individually containing the H. ducreyi cdtA and cdtC genes, we purified a noncovalent CdtA-CdtC complex. Incubation of this CdtA-CdtC complex with HeLa cells blocked killing of these cells by CDT holotoxin. Furthermore, the addition of purified recombinant CdtB to HeLa cells preincubated with the CdtA-CdtC complex resulted in the killing of these human epithelial cells.

引用

页码：6633 / 6640

页数：8

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