Flexible FET-Type VEGF Aptasensor Based on Nitrogen-Doped Graphene Converted from Conducting Polymer

被引：208

作者：

Kwon, Oh Seok ^{[2
]}

Park, Seon Joo ^{[2
]}

Hong, Jin-Yong ^{[2
]}

Han, A-Reum ^{[1
]}

Lee, Jun Seop ^{[2
]}

Lee, James S. ^{[2
]}

Oh, Joon Hak ^{[1
]}

Jang, Jyongsik ^{[2
]}

机构：

[1] Ulsan Natl Inst Sci & Technol, Sch Nanobiosci & Chem Engn, KIER UNIST Adv Ctr Energy, Low Dimens Carbon Mat Ctr, Ulsan 689798, South Korea

[2] Seoul Natl Univ, Sch Chem & Biol Engn, World Class Univ Program Chem Convergence Energy, Seoul 151742, South Korea

来源：

ACS NANO | 2012年 / 6卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

nitrogen-doped graphene; field-effect transistor; conducting polymer; aptasensor; vascular endothelial growth factor; aptamer; CHEMICAL-VAPOR-DEPOSITION; ENDOTHELIAL GROWTH-FACTOR; CARBON NANOTUBE; LAYER GRAPHENE; FABRICATION; NANOMATERIALS; BIOSENSORS; PLATFORM; SHEETS; LIQUID;

D O I：

10.1021/nn204395n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene-based field-effect transistors (FETs) have been developed rapidly and are currently considered as an alternative for postsilicon electronics. In this study, polypyrrole-converted nitrogen-doped few-layer graphene (PPy-NDFLG) was grown on Cu substrate by chemical vapor deposition combined with vapor deposition polymerization and then transferred onto a flexible substrate. Furthermore, antivascular endothelial growth factor (VEGF) RNA aptamer conjugated PPy-NDFLG was integrated Into a liquid-ion gated FET geometry to fabricate a high-performance VEGF aptamer-based sensor. Field-induced high sensitivity was observed for the analyte-binding events, eventually leading to the recognition of the target molecules at an unprecedentedly low concentration (100 fM). Additionally, the aptasensor had excellent reusability, mechanical bendability, and durability In the flexible process. The developed methodology describes, for the first time, the fabrication of N-doped graphene using conducting polymers including heteroatoms in their structures as the carbonization precursor and demonstrates its use in a high-performance, flexible FET-type aptasensor to detect vascular endothelial growth factor as a cancer biomarker.

引用

页码：1486 / 1493

页数：8