Flexible FET-Type VEGF Aptasensor Based on Nitrogen-Doped Graphene Converted from Conducting Polymer

被引:208
作者
Kwon, Oh Seok [2 ]
Park, Seon Joo [2 ]
Hong, Jin-Yong [2 ]
Han, A-Reum [1 ]
Lee, Jun Seop [2 ]
Lee, James S. [2 ]
Oh, Joon Hak [1 ]
Jang, Jyongsik [2 ]
机构
[1] Ulsan Natl Inst Sci & Technol, Sch Nanobiosci & Chem Engn, KIER UNIST Adv Ctr Energy, Low Dimens Carbon Mat Ctr, Ulsan 689798, South Korea
[2] Seoul Natl Univ, Sch Chem & Biol Engn, World Class Univ Program Chem Convergence Energy, Seoul 151742, South Korea
基金
新加坡国家研究基金会;
关键词
nitrogen-doped graphene; field-effect transistor; conducting polymer; aptasensor; vascular endothelial growth factor; aptamer; CHEMICAL-VAPOR-DEPOSITION; ENDOTHELIAL GROWTH-FACTOR; CARBON NANOTUBE; LAYER GRAPHENE; FABRICATION; NANOMATERIALS; BIOSENSORS; PLATFORM; SHEETS; LIQUID;
D O I
10.1021/nn204395n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Graphene-based field-effect transistors (FETs) have been developed rapidly and are currently considered as an alternative for postsilicon electronics. In this study, polypyrrole-converted nitrogen-doped few-layer graphene (PPy-NDFLG) was grown on Cu substrate by chemical vapor deposition combined with vapor deposition polymerization and then transferred onto a flexible substrate. Furthermore, antivascular endothelial growth factor (VEGF) RNA aptamer conjugated PPy-NDFLG was integrated Into a liquid-ion gated FET geometry to fabricate a high-performance VEGF aptamer-based sensor. Field-induced high sensitivity was observed for the analyte-binding events, eventually leading to the recognition of the target molecules at an unprecedentedly low concentration (100 fM). Additionally, the aptasensor had excellent reusability, mechanical bendability, and durability In the flexible process. The developed methodology describes, for the first time, the fabrication of N-doped graphene using conducting polymers including heteroatoms in their structures as the carbonization precursor and demonstrates its use in a high-performance, flexible FET-type aptasensor to detect vascular endothelial growth factor as a cancer biomarker.
引用
收藏
页码:1486 / 1493
页数:8
相关论文
共 71 条
[11]   Raman spectrum of graphene and graphene layers [J].
Ferrari, A. C. ;
Meyer, J. C. ;
Scardaci, V. ;
Casiraghi, C. ;
Lazzeri, M. ;
Mauri, F. ;
Piscanec, S. ;
Jiang, D. ;
Novoselov, K. S. ;
Roth, S. ;
Geim, A. K. .
PHYSICAL REVIEW LETTERS, 2006, 97 (18)
[12]  
Foekens JA, 2001, CANCER RES, V61, P5407
[13]   A Dual Platform for Selective Analyte Enrichment and Ionization in Mass Spectrometry Using Aptamer-Conjugated Graphene Oxide [J].
Gulbakan, Basri ;
Yasun, Emir ;
Shukoor, M. Ibrahim ;
Zhu, Zhi ;
You, Mingxu ;
Tan, Xiaohong ;
Sanchez, Hernan ;
Powell, David H. ;
Dai, Hongjie ;
Tan, Weihong .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (49) :17408-17410
[14]   Controllable N-Doping of Graphene [J].
Guo, Beidou ;
Liu, Qian ;
Chen, Erdan ;
Zhu, Hewei ;
Fang, Liang ;
Gong, Jian Ru .
NANO LETTERS, 2010, 10 (12) :4975-4980
[15]   Influence of Electrolyte Composition on Liquid-Gated Carbon Nanotube and Graphene Transistors [J].
Heller, Iddo ;
Chatoor, Sohail ;
Mannik, Jaan ;
Zevenbergen, Marcel A. G. ;
Dekker, Cees ;
Lemay, Serge G. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (48) :17149-17156
[16]   A strategy for fabricating single layer graphene sheets based on a layer-by-layer self-assembly [J].
Hong, Jin-Yong ;
Shin, Keun-Young ;
Kwon, Oh Seok ;
Kang, Haeyoung ;
Jang, Jyongsik .
CHEMICAL COMMUNICATIONS, 2011, 47 (25) :7182-7184
[17]   Facile fabrication of inorganic-polymer core-shell nanostructures by a one-step vapor deposition polymerization [J].
Jang, J ;
Lim, B .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (45) :5600-5603
[18]  
Jang J, 2002, ANGEW CHEM INT EDIT, V41, P4016, DOI 10.1002/1521-3773(20021104)41:21<4016::AID-ANIE4016>3.0.CO
[19]  
2-G
[20]   Fabrication of magnetic carbon nanotubes using a metal-impregnated polymer precursor [J].
Jang, JS ;
Yoon, HS .
ADVANCED MATERIALS, 2003, 15 (24) :2088-+