Comparative study of the LC-MS/MS and UPLC-MS/MS for the multi-residue analysis of quinolones, penicillins and cephalosporins in cow milk, and validation according to the regulation 2002/657/EC

被引：93

作者：

Junza, A. ^{[1
]}

Amatya, R. ^{[1
]}

Barron, D. ^{[1
]}

Barbosa, J. ^{[1
]}

机构：

[1] Univ Barcelona, Dept Analyt Chem, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2011年 / 879卷 / 25期

关键词：

Antibiotics; Milk; LC-MS/MS; UPLC-MS/MS; Matrix effect; Validation; TANDEM MASS-SPECTROMETRY; BETA-LACTAM ANTIBIOTICS; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; ELECTROSPRAY-IONIZATION; BOVINE-MILK; VETERINARY DRUGS; CONFIRMATION; PERFORMANCE; COMPENSATE;

D O I：

10.1016/j.jchromb.2011.07.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to develop and validate an analytical method to simultaneously determine European Union-regulated beta-lactams (penicillins and cephalosporins) and quinolones in cow milk. The procedure involves a new solid phase extraction (SPE) to clean-up and pre-concentrate the three series of antibiotics before analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). LC-MS/MS and UPLC-MS/MS techniques were also compared. The method was validated according to the Directive 2002/657/EC and subsequently applied to 56 samples of raw cow milk supplied by the Laboratori Interprofessional Lleter de Catalunya (ALLIC) (Laboratori Interprofessional Lleter de Catalunya, Control Laboratory Interprofessional of Milk of Catalunya). (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：2601 / 2610

页数：10

共 29 条

[1] Multi-residue determination of veterinary drugs in milk by ultra-high-pressure liquid chromatography-tandem mass spectrometry [J].