Label-Free Immunochemistry Approach to Detect and Identity Antibiotics in Milk

The rise of antibiotic-resistant bacteria is a major concern for the continued health and well-being of the general population. The widespread use of antibiotics within the farming industry is one of the factors that have been linked to the appearance of these resistant strains. Regulation exists to prevent antibiotic foodstuffs such as milk being distributed to consumers; however, for this to be effective, methods must exist for testing the milk to enforce these regulations. Current techniques are often time consuming and expensive, especially when applied to large number of samples. This has led to an interest in milk analyses, which can be carried out outside the laboratory. The development of immunosensors that exploit the exquisite specificity of antibody binding has been studied, both using labeled antibodies such as ELISA assays and, more recently, label-free approaches that directly detect the presence of the antibiotic on binding to a specific antibody. Within this review, we detail recent advances in the detection of antibiotics, especially in milk, using a variety of methods. Various techniques such as electrochemical and surface plasmon resonance methods are described. (Pediatr Res 67: 476-480, 2010)