Screen-printed biosensors using different alcohol oxidases

被引:84
作者
Patel, NG
Meier, S
Cammann, K
Chemnitius, GC
机构
[1] ICB, D-48149 Munster, Germany
[2] Univ Munster, Lehrstuhl Analyt Chem, D-48149 Munster, Germany
[3] Sardar Patel Univ, SICART, Dept Elect, Vallabh Vidyanagar 388120, Gujarat, India
关键词
ethanol; enzyme sensor; alcohol oxidase; screen-printing; wine analysis;
D O I
10.1016/S0925-4005(01)00545-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Low-cost screen-printed sensors consisting of a platinum working electrode, a carbon counter electrode, and an Ag/AgCl pseudo-reference electrode were fabricated for the development of alcohol oxidase biosensors. The sensors were fabricated as amperometric transducers for the detection of alcohols in batch systems. A mixture of alcohol oxidase (AOD) with poly(carbamoyl)sulfonate (PCS) hydrogel was used for enzyme immobilization onto the platinum electrodes. Alcohol oxidases from different sources such as from Hansenula sp., from Candida boidinii and from Pichia pastoris were used for immobilization. The performances of the resulting different sensors has been compared and characterized with respect to enzyme load, pH and temperature dependence, response time, recovery time, linear range and sensitivity. The relative response of sensors for different alcohols was measured to evaluate the selectivity of the sensors. The effect of ascorbic acid and sodium sulfite as electrochemical interferents on the sensor's performance was investigated. The continuous operation and storage stability of the sensors were also evaluated. Most of the characterization parameters were found to be superior for sensors with immobilized AOD from Hansenula sp, The sensors were also tested with wine samples. The results obtained by the newly developed biosensors were compared to results obtained by pycnometry, the well-established reference method as well as gas chromatograpty method. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:101 / 110
页数:10
相关论文
共 40 条
  • [1] ALVAREZICAZA M, 1993, ANAL CHEM, V65, P525
  • [2] Simultaneous enzymatic spectrophotometric determination of ethanol and methanol by use of artificial neural networks for calibration
    Blanco, M
    Coello, J
    Iturriaga, H
    Maspoch, S
    Porcel, M
    [J]. ANALYTICA CHIMICA ACTA, 1999, 398 (01) : 83 - 92
  • [3] Development of a disposable ethanol biosensor based on a chemically modified screen-printed electrode coated with alcohol oxidase for the analysis of beer
    Boujtita, M
    Hart, JP
    Pittson, R
    [J]. BIOSENSORS & BIOELECTRONICS, 2000, 15 (5-6) : 257 - 263
  • [4] Determination of the concentration of higher alcohols with enzyme coupled flow-injection analysis in model systems
    Burfeind, J
    Weigel, B
    Kretzmer, G
    Schugerl, K
    Huwig, A
    Giffhorn, F
    [J]. ANALYTICA CHIMICA ACTA, 1996, 322 (03) : 131 - 139
  • [5] MONITORING OF ETHANOL DURING FERMENTATION OF A LIGNOCELLULOSE HYDROLYSATE BY ONLINE MICRODIALYSIS SAMPLING, COLUMN LIQUID-CHROMATOGRAPHY, AND AN ALCOHOL BIOSENSOR
    BUTTLER, T
    GORTON, L
    JARSKOG, H
    MARKOVARGA, G
    HAHNHAGERDAL, B
    MEINANDER, N
    OLSSON, L
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1994, 44 (03) : 322 - 328
  • [6] ELECTROCATALYTIC OXIDATION OF REDUCED NICOTINAMIDE COENZYMES AT METHYLENE GREEN-MODIFIED ELECTRODES AND FABRICATION OF AMPEROMETRIC ALCOHOL BIOSENSORS
    CHI, QJ
    DONG, SJ
    [J]. ANALYTICA CHIMICA ACTA, 1994, 285 (1-2) : 125 - 133
  • [7] CRIDDLE WJ, 1995, ETHANOL ENCY ANAL SC, V2
  • [8] ELMER P, ANAL INSTRUMENTS ALC
  • [9] Flexible amperometric transducers for biosensors based on a screen printed three electrode system
    Erlenkötter, A
    Kottbus, M
    Chemnitius, GC
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2000, 481 (01): : 82 - 94
  • [10] FREBEL H, 1999, THESIS U MUNSTER GER