Overexpression of acetyl-CoA synthetase in Saccharomyces cerevisiae increases acetic acid tolerance

被引：58

作者：

Ding, Jun ^{[1
,2
,3
]}

Holzwarth, Garrett ^{[4
]}

Penner, Michael H. ^{[2
]}

Patton-Vogt, Jana ^{[5
]}

Bakalinsky, Alan T. ^{[1
,2
]}

机构：

[1] Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA

[2] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA

[3] Res & Dev Medolac Labs, Lake Oswego, OR 97035 USA

[4] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA

[5] Duquesne Univ, Dept Biol Sci, Pittsburgh, PA 15282 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2015年 / 362卷 / 03期

基金：

美国农业部; 美国国家卫生研究院;

关键词：

Saccharomyces cerevisiae; acetyl-CoA synthetase; Acs2; acetic acid; lignocellulose; COENZYME-A SYNTHETASE; PYRUVATE-DEHYDROGENASE BYPASS; PROGRAMMED CELL-DEATH; YEAST; ACETATE; GROWTH; GENE; GLUCOSE; INVOLVEMENT; INHIBITION;

D O I：

10.1093/femsle/fnu042

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Acetic acid-mediated inhibition of the fermentation of lignocellulose-derived sugars impedes development of plant biomass as a source of renewable ethanol. In order to overcome this inhibition, the capacity of Saccharomyces cerevisiae to synthesize acetyl-CoA from acetic acid was increased by overexpressing ACS2 encoding acetyl-coenzyme A synthetase. Overexpression of ACS2 resulted in higher resistance to acetic acid as measured by an increased growth rate and shorter lag phase relative to a wild-type control strain, suggesting that Acs2-mediated consumption of acetic acid during fermentation contributes to acetic acid detoxification.

引用

页数：7

共 39 条

[21]

Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae [J].