Efficiency of the hybrid capture 2 HPV DNA test in cervical cancer screening -: A study by the french society of clinical cytology

被引:65
作者
de Cremoux, P
Coste, J
Sastre-Garau, X
Thioux, M
Bouillac, C
Labbé, S
Cartier, I
Ziol, M
Dosda, A
Le Galès, C
Molinié, V
Vacher-Lavenu, MC
Cochand-Priollet, B
Vielh, P
Magdelénat, H
机构
[1] Inst Curie, Dept Tumor Biol, F-75248 Paris, France
[2] Hop Cochin, Dept Biostat, AP, HP, F-75674 Paris, France
[3] Univ Paris 05, F-75270 Paris 06, France
[4] Pathol Ctr, La Rochelle, France
[5] Pathol Ctr Dr R Cartier, Paris, France
[6] Univ Paris 13, Jean Verdier Hosp, Dept Pathol, Bondy, France
[7] Hop Louis Mourier, Dept Pathol, F-92701 Colombes, France
[8] CNRS, UPRESA 80 85, INSERM, U537,Ctr Hlth Econ & Adm Res, Le Kremlin Bicetre, France
[9] Hop Foch, Dept Pathol, Suresnes, France
[10] Hop Cochin, AP, HP, Dept Pathol, F-75674 Paris, France
[11] Hop Lariboisiere, AP, HP, Dept Pathol, F-75475 Paris, France
关键词
cervical cancer; screening; human papillomavirus; hybrid Capture 2 test;
D O I
10.1309/XFUCPP6M5XUA94B8
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The aim of this study was to determine the efficiency of the Hybrid Capture 2 (HC2; Digene, Gaithersburg, MD) human papillomavirus (HPV) assay for the detection of cervical neoplasia. Of the 1, 785 patients recruited, 462 (25.88%) were referred for colposcopy owing to previously detected cytologic abnormalities, and 1,323 (74.12%) were voluntary candidates for screening. For all patients, a Papanicolaou smear and a monolayer smear (ThinPrep, Cytyc, Boxborough, MA) were done. HPV DNA was detected on the residual liquid-based material. False-positive results were observed in 111 cases and comprised 34 cross-reactions (1.90%) and 77 false-positive cases (4.31%) owing to a contiguous strong chemiluminescence signal. Interestingly, all these samples had a relative light units value of 1 to 3 and were contiguous to a sample with a very high HPV DNA load. The final results showed that high-risk and low-risk HPV DNA were detected in 480 samples (26.89%) and 135 samples (7.56%), respectively. Although HC2 can be considered a reliable and sensitive test for HPV DNA detection, we do not advocate its use for large-scale screening for cervical neoplasia.
引用
收藏
页码:492 / 499
页数:8
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