A PCR-based assay to detect hAT-like transposon sequences in plants

被引：7

作者：

De Keukeleire, P

De Schepper, S

Gielis, J

Gerats, T

机构：

[1] State Univ Ghent, Fac Sci, Dept Plant Syst Biol, B-9000 Ghent, Belgium

[2] Geniaal Bvba, B-2018 Antwerp, Belgium

[3] State Univ Ghent, Fac Appl Biol & Agr Sci, Dept Plant Prod, B-9000 Ghent, Belgium

来源：

CHROMOSOME RESEARCH | 2004年 / 12卷 / 02期

关键词：

hAT-like transposon; phylogeny; plant genomics; polymerase chain reaction (PCR);

D O I：

10.1023/B:CHRO.0000013163.34505.96

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite their potential as endogenous tools for forward and reverse genetics, members of the hobo, Ac, Tam3 (or hAT) superfamily of transposable elements have been characterized in but a limited number of plant species. To expedite their isolation, we developed a PCR-based assay for the detection of hAT-like transposon sequences in plants which was applied to isolate and initially characterize such sequences from Petunia hybrida, Phaseolus vulgaris, Bambusa vulgaris, Brassica napus and Rhododendron simsii.

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收藏

页码：117 / 123

页数：7

相关论文

共 31 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] THE HOBO TRANSPOSABLE ELEMENT OF DROSOPHILA CAN BE CROSS-MOBILIZED IN HOUSEFLIES AND EXCISES LIKE THE AC ELEMENT OF MAIZE [J].

ATKINSON, PW ;

WARREN, WD ;

OBROCHTA, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9693-9697

[3] TRANSPOSITION OF THE MAIZE CONTROLLING ELEMENT ACTIVATOR IN TOBACCO [J].

BAKER, B ;

SCHELL, J ;

LORZ, H ;

FEDOROFF, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (13) :4844-4848

[4]

Capy P, 1996, J MOL EVOL, V42, P359, DOI 10.1007/BF02337546

[5] TRANSPOSABLE ELEMENTS IN LEPIDOPTERA - HOBO-LIKE TRANSPOSONS IN HELIOTHIS-VIRESCENS AND HELICOVERPA-ZEA [J].

DEVAULT, JD ;

NARANG, SK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 203 (01) :169-175

[6] A novel pseudoautosomal human gene encodes a putative protein similar to Ac-like transposases [J].

Esposito, T ;

Gianfrancesco, F ;

Ciccodicola, A ;

Montanini, L ;

Mumm, S ;

D'Urso, M ;

Forabosco, A .

HUMAN MOLECULAR GENETICS, 1999, 8 (01) :61-67

[7] ISOLATION OF THE TRANSPOSABLE MAIZE CONTROLLING ELEMENTS AC AND DS [J].

FEDOROFF, N ;

WESSLER, S ;

SHURE, M .

CELL, 1983, 35 (01) :235-242

[8] Molecular and functional characterization of Slide, an Ac-like autonomous transposable element from tobacco [J].

Grappin, P ;

Audeon, C ;

Chupeau, MC ;

Grandbastien, MA .

MOLECULAR & GENERAL GENETICS, 1996, 252 (04) :386-397

[9] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[10]

Handler AM, 1996, GENETICS, V143, P1339

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