Immunization of dogs and cats with a DNA vaccine against rabies virus

被引：77

作者：

Osorio, JE ^{[1
]}

Tomlinson, CC ^{[1
]}

Frank, RS ^{[1
]}

Haanes, EJ ^{[1
]}

Rushlow, K ^{[1
]}

Haynes, JR ^{[1
]}

Stinchcomb, DT ^{[1
]}

机构：

[1] Heska Corp, Ft Collins, CO 80525 USA

来源：

VACCINE | 1999年 / 17卷 / 9-10期

关键词：

DNA vaccines; rabies; dogs; cats; mice;

D O I：

10.1016/S0264-410X(98)00328-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The applicability of DNA immunization technology for vaccine development in companion animals was investigated by immunizing dogs and cats by the intramuscular (i.m.) and intradermal (i.d.) routes with a plasmid DNA vector encoding the rabies virus glycoprotein G. In dogs, administration of 100 mu g DNA doses by the i.m. route resulted in stronger and more durable rabies virus neutralizing antibody (RVNA) titers than those obtained by i.d. inoculation. In contrast, i.m. vaccination of cats with a similar dose was less effective in terms of mean titer and seroconversion frequency. However, efficacy was improved by increasing the dosage to 300 mu g of DNA per immunization. Interestingly, i.d. inoculation of cats appeared to be a superior route of delivery in this species, resulting in higher seroconversion frequency than i.m. administration. In addition, geometric mean RVNA titers in i.d. inoculated cats increased over four-fold during a seven month period following a second and final immunization. These results demonstrate that non-facilitated, naked DNA vaccines can elicit strong, antigen-specific immune responses in dogs and cats, and DNA immunization may be a useful tool for future development of novel vaccines for these species. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1109 / 1116

页数：8

共 41 条

[21] Immunization of pigs with a particle-mediated DNA vaccine to influenza A virus protects against challenge with homologous virus [J].