Sub-ppb level detection of 2-hydroxybiphenyl using portable surface-plasmon-resonance immunosensor

被引：12

作者：

Gobi, KV ^{[1
]}

Kim, SJ ^{[1
]}

Harada, R ^{[1
]}

Miura, N ^{[1
]}

机构：

[1] Kyushu Univ, Adv Sci & Technol Ctr Cooperat Res, Kasuga, Fukuoka 8168580, Japan

来源：

ELECTROCHEMISTRY | 2003年 / 71卷 / 06期

关键词：

surface plasmon resonance; SPR sensor; 2-hydroxybiphenyl; immunosensor; pepsin;

D O I：

10.5796/electrochemistry.71.430

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A sensor-chip-module integrated portable surface-plasmon-resonance (SPR) immunosensor was employed for monitoring the indirect competitive immunoreaction of the monoclonal antibody against 2-hydroxybiphenyl between the analyte (2-hydroxybiphenyl, abbreviated as HBP hereafter) in test solution and the HBP-protein conjugate immobilized on sensor chip. This sensor was found to detect HBP rather sensitively in the concentration range of 0.1-1000 ppb. The sensor chip could be reusable several times by removing the antibody anchored to the sensor chip on treatment with a pepsin solution (pH 2.0) for a few minutes.

引用

页码：430 / 432

页数：3

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