Sub-ppb level detection of 2-hydroxybiphenyl using portable surface-plasmon-resonance immunosensor

被引:12
作者
Gobi, KV [1 ]
Kim, SJ [1 ]
Harada, R [1 ]
Miura, N [1 ]
机构
[1] Kyushu Univ, Adv Sci & Technol Ctr Cooperat Res, Kasuga, Fukuoka 8168580, Japan
关键词
surface plasmon resonance; SPR sensor; 2-hydroxybiphenyl; immunosensor; pepsin;
D O I
10.5796/electrochemistry.71.430
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
A sensor-chip-module integrated portable surface-plasmon-resonance (SPR) immunosensor was employed for monitoring the indirect competitive immunoreaction of the monoclonal antibody against 2-hydroxybiphenyl between the analyte (2-hydroxybiphenyl, abbreviated as HBP hereafter) in test solution and the HBP-protein conjugate immobilized on sensor chip. This sensor was found to detect HBP rather sensitively in the concentration range of 0.1-1000 ppb. The sensor chip could be reusable several times by removing the antibody anchored to the sensor chip on treatment with a pepsin solution (pH 2.0) for a few minutes.
引用
收藏
页码:430 / 432
页数:3
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