Co does not replace Ni with respect to urease activity in zucchini (Cucurbita pepo convar. giromontiina) and soybean (Glycine max)

被引:12
作者
Gerendás, J [1 ]
Polacco, JC
Freyermuth, SK
Sattelmacher, B
机构
[1] Univ Kiel, Inst Plant Nutr & Soil Sci, D-24118 Kiel, Germany
[2] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA
关键词
cobalt; nickel; soybean; substitution; urease activity; zucchini;
D O I
10.1023/A:1004343416511
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
In order to evaluate whether Co can functionally replace Ni in urease, as suggested by Watanabe et al. (Soil Sci. Plant Nutr. (1994) 40, 545-548), zucchini and soybean plants were grown in purified nutrient solutions containing nitrate and ammonium as sole N source supplemented either with 0.05 mmol m(-3) Ni or Co or with no supplementation (control). In addition, isolated soybean cotyledons were incubated axenically with either 1 mmol m(-3) Ni or Co supplements. Plant growth was not affected by the Ni and Co additions, but urease activity was only detected in Ni-supplemented tissue, and in no case did Co raise the urease activity level. Only Ni-deprived plants (-Ni - Co and -Ni + Co) accumulated appreciable amounts of urea in the leaves. These results strongly suggest, that Co does not replace Ni with respect to functional urease.
引用
收藏
页码:127 / 135
页数:9
相关论文
共 33 条
  • [1] [Anonymous], 1997, 11466 DIN ISO
  • [2] JACK-BEAN-UREASE - THE 1ST NICKEL ENZYME
    BLAKELEY, RL
    ZERNER, B
    [J]. JOURNAL OF MOLECULAR CATALYSIS, 1984, 23 (2-3): : 263 - 292
  • [3] BENEFICIAL-EFFECTS OF NICKEL ON PLANT-GROWTH
    BROWN, PH
    WELCH, RM
    CARY, EE
    CHECKAI, RT
    [J]. JOURNAL OF PLANT NUTRITION, 1987, 10 (9-16) : 2125 - 2135
  • [4] NICKEL IN PLANTS .2. DISTRIBUTION AND CHEMICAL FORM IN SOYBEAN PLANTS
    CATALDO, DA
    GARLAND, TR
    WILDUNG, RE
    DRUCKER, H
    [J]. PLANT PHYSIOLOGY, 1978, 62 (04) : 566 - 570
  • [5] JACK BEAN UREASE (EC 3.5.1.5) .2. THE RELATIONSHIP BETWEEN NICKEL, ENZYMATIC-ACTIVITY, AND THE ABNORMAL ULTRAVIOLET-SPECTRUM - THE NICKEL CONTENT OF JACK BEANS
    DIXON, NE
    GAZZOLA, C
    ASHER, CJ
    LEE, DSW
    BLAKELEY, RL
    ZERNER, B
    [J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1980, 58 (06): : 474 - 480
  • [6] JACK BEAN UREASE (EC 3.5.1.5) .1. A SIMPLE DRY ASHING PROCEDURE FOR THE MICRO-DETERMINATION OF TRACE-METALS IN PROTEINS - THE NICKEL CONTENT OF UREASE
    DIXON, NE
    BLAKELEY, RL
    ZERNER, B
    [J]. CANADIAN JOURNAL OF BIOCHEMISTRY, 1980, 58 (06): : 469 - 473
  • [7] NICKEL - AN ESSENTIAL MICRONUTRIENT FOR LEGUMES AND POSSIBLY ALL HIGHER-PLANTS
    ESKEW, DL
    WELCH, RM
    CARY, EE
    [J]. SCIENCE, 1983, 222 (4624) : 621 - 623
  • [8] NICKEL IN HIGHER-PLANTS - FURTHER EVIDENCE FOR AN ESSENTIAL ROLE
    ESKEW, DL
    WELCH, RM
    NORVELL, WA
    [J]. PLANT PHYSIOLOGY, 1984, 76 (03) : 691 - 693
  • [9] Significance of Ni supply for growth, urease activity and the concentrations of urea, amino acids and mineral nutrients of urea-grown plants
    Gerendas, J
    Sattelmacher, B
    [J]. PLANT AND SOIL, 1997, 190 (01) : 153 - 162
  • [10] Significance of N source (urea vs. NH4NO3) and Ni supply for growth, urease activity and nitrogen metabolism of zucchini (Cucurbita pepo convar. giromontiina)
    Gerendas, J
    Sattelmacher, B
    [J]. PLANT AND SOIL, 1997, 196 (02) : 217 - 222