Capillary electrophoresis coupled to mass spectrometry for clinical diagnostic purposes

被引:79
作者
Fliser, D
Wittke, S
Mischak, H
机构
[1] Hannover Med Sch, D-30625 Hannover, Germany
[2] Mosa Diagnost & Therapeut, Hannover, Germany
关键词
biomarkers; capillary electrophoresis; mass spectrometry; peptides; review; urine;
D O I
10.1002/elps.200500187
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The introduction of fast, sensitive, and robust techniques for proteomic analysis into clinical practice represents a major step toward a new diagnostic approach of body fluids. In addition, proteomics emerges as a key technology for the discovery of disease biomarkers in various body fluids. However, even in relatively protein-deprived body fluids such as urine, the complexity and wide dynamic range of protein expression pose a considerable challenge to both separation and identification technologies. In the present review we discuss from a clinical point-of-view recent advances of the use of proteomics in clinical diagnosis as well as therapy evaluation. We focus on capillary electrophoresis coupled to mass spectrometry (CE-MS) and discuss CE-MS from an application point of view evaluating its merits and vices with regard to biomarker discovery. This review further presents examples of clinical applications of CE-MS for detection and identification of biomarkers in urine.
引用
收藏
页码:2708 / 2716
页数:9
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