Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications

被引：3351

作者：

Krueger, Felix ^{[1
]}

Andrews, Simon R. ^{[1
]}

机构：

[1] Babraham Inst, Bioinformat Grp, Cambridge CB22 3AT, England

来源：

BIOINFORMATICS | 2011年 / 27卷 / 11期

基金：

英国生物技术与生命科学研究理事会;

关键词：

DNA METHYLATION; GENOME; ANIMALS; PLANTS;

D O I：

10.1093/bioinformatics/btr167

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A combination of bisulfite treatment of DNA and high-throughput sequencing (BS-Seq) can capture a snapshot of a cell's epigenomic state by revealing its genome-wide cytosine methylation at single base resolution. Bismark is a flexible tool for the time-efficient analysis of BS-Seq data which performs both read mapping and methylation calling in a single convenient step. Its output discriminates between cytosines in CpG, CHG and CHH context and enables bench scientists to visualize and interpret their methylation data soon after the sequencing run is completed.

引用

页码：1571 / 1572

页数：2

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