Catalposide protects Neuro 2A cells from hydrogen peroxide-induced cytotoxicity via the expression of heme oxygenase-1

被引：35

作者：

Moon, MK

Choi, BM

Oh, GS

Pae, HO

Kim, JD

Oh, H

Oh, CS

Kim, DH

Rho, YD

Shin, MK

Lee, HS

Chung, HT ^{[1
]}

机构：

[1] Wonkwang Univ, Sch Med, Med Resources Res Ctr, Iksan 570749, Chonbuk, South Korea

[2] Wonkwang Univ, Sch Med, Dept Microbiol & Immunol, Iksan 570749, Chonbuk, South Korea

[3] Wonkwang Univ, Sch Med, Dept Pediat, Chonbuk 570749, South Korea

[4] Profess Grad Sch Oriental Med, Chonbuk 570749, South Korea

[5] Wonkwang Univ, Coll Oriental Med, Chonbuk 570749, South Korea

来源：

TOXICOLOGY LETTERS | 2003年 / 145卷 / 01期

关键词：

carbon monoxide; catalposide; herne oxygenase; hydrogen peroxide; Neuro 2A cells;

D O I：

10.1016/S0378-4274(03)00268-6

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniaceae) has been shown to possess anti-microbial, anti-tumoral, and anti-inflammatory properties. Heme oxygenase-1 (HO-1) is a stress response protein and is known to play a protective role against the oxidative injury. In this study, we examined whether catalposide could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 protein expression and HO activity. The treatment of the cells with catalposide resulted in dose- and time-dependent up-regulations of both HO-1 protein expression and HO activity. Catalposide protected the cells from hydrogen peroxide-induced cell death. The protective effect of catalposide on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. Additional experiments revealed the involvement of CO in the cytoprotective effect of catalposide-induced HO-1. These results indicate that catalposide is a potent inducer of HO-1 and HO-1 induction is responsible for the catalposide-mediated cytoprotection against oxidative damage. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：46 / 54

页数：9

共 22 条

[1]

Inhibition of TNF-α, IL-1β, and IL-6 productions and NF-κB activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated ftom Catalpa ovata G. Don (Bignoniaceae) [J].