Catalposide protects Neuro 2A cells from hydrogen peroxide-induced cytotoxicity via the expression of heme oxygenase-1

被引:35
作者
Moon, MK
Choi, BM
Oh, GS
Pae, HO
Kim, JD
Oh, H
Oh, CS
Kim, DH
Rho, YD
Shin, MK
Lee, HS
Chung, HT [1 ]
机构
[1] Wonkwang Univ, Sch Med, Med Resources Res Ctr, Iksan 570749, Chonbuk, South Korea
[2] Wonkwang Univ, Sch Med, Dept Microbiol & Immunol, Iksan 570749, Chonbuk, South Korea
[3] Wonkwang Univ, Sch Med, Dept Pediat, Chonbuk 570749, South Korea
[4] Profess Grad Sch Oriental Med, Chonbuk 570749, South Korea
[5] Wonkwang Univ, Coll Oriental Med, Chonbuk 570749, South Korea
关键词
carbon monoxide; catalposide; herne oxygenase; hydrogen peroxide; Neuro 2A cells;
D O I
10.1016/S0378-4274(03)00268-6
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 [卫生毒理学];
摘要
Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don (Bignoniaceae) has been shown to possess anti-microbial, anti-tumoral, and anti-inflammatory properties. Heme oxygenase-1 (HO-1) is a stress response protein and is known to play a protective role against the oxidative injury. In this study, we examined whether catalposide could protect Neuro 2A cells, a kind of neuronal cell lines, from oxidative damage through the induction of HO-1 protein expression and HO activity. The treatment of the cells with catalposide resulted in dose- and time-dependent up-regulations of both HO-1 protein expression and HO activity. Catalposide protected the cells from hydrogen peroxide-induced cell death. The protective effect of catalposide on hydrogen peroxide-induced cell death was abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. Additional experiments revealed the involvement of CO in the cytoprotective effect of catalposide-induced HO-1. These results indicate that catalposide is a potent inducer of HO-1 and HO-1 induction is responsible for the catalposide-mediated cytoprotection against oxidative damage. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:46 / 54
页数:9
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