Cryptosporidium parvum Cpn60 targets a relict organelle

被引：60

作者：

Riordan, CE

Ault, JG

Langreth, SG

Keithly, JS

机构：

[1] New York State Dept Hlth, Wadsworth Ctr, Axelrod Inst Publ Hlth, Albany, NY 12208 USA

[2] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY 12208 USA

[3] Uniformed Serv Univ Hlth Sci, Dept Microbiol & Immunol, Bethesda, MD 20814 USA

来源：

CURRENT GENETICS | 2003年 / 44卷 / 03期

关键词：

apicomplexan; relict mitochondrion; chaperonin 60 (Cpn60);

D O I：

10.1007/s00294-003-0432-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites (CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.

引用

页码：138 / 147

页数：10

共 60 条

[11] Cryptosporidium is more closely related to the gregarines than to coccidia as shown by phylogenetic analysis of apicomplexan parasites inferred using small-subunit ribosomal RNA gene sequences [J].