Cryptosporidium parvum Cpn60 targets a relict organelle

被引：60

作者：

Riordan, CE

Ault, JG

Langreth, SG

Keithly, JS

机构：

[1] New York State Dept Hlth, Wadsworth Ctr, Axelrod Inst Publ Hlth, Albany, NY 12208 USA

[2] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY 12208 USA

[3] Uniformed Serv Univ Hlth Sci, Dept Microbiol & Immunol, Bethesda, MD 20814 USA

来源：

CURRENT GENETICS | 2003年 / 44卷 / 03期

关键词：

apicomplexan; relict mitochondrion; chaperonin 60 (Cpn60);

D O I：

10.1007/s00294-003-0432-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites (CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.

引用

收藏

页码：138 / 147

页数：10

相关论文

共 60 条

[21] MITOCHONDRIA OF MAMMALIAN PLASMODIUM SPP [J].

FRY, M ;

BEESLEY, JE .

PARASITOLOGY, 1991, 102 :17-26

[22] EVOLUTION OF THE CHAPERONIN FAMILIES (HSP60, HSP10 AND TCP-1) OF PROTEINS AND THE ORIGIN OF EUKARYOTIC CELLS [J].

GUPTA, RS .

MOLECULAR MICROBIOLOGY, 1995, 15 (01) :1-11

[23] Secondary absence of mitochondria in Giardia lamblia and Trichomonas vaginalis revealed by valyl-tRNA synthetase phylogeny [J].

Hashimoto, T ;

Sanchez, LB ;

Shirakura, T ;

Müller, M ;

Hasegawa, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (12) :6860-6865

[24] HOMOLOGOUS PLANT AND BACTERIAL PROTEINS CHAPERONE OLIGOMERIC PROTEIN ASSEMBLY [J].

HEMMINGSEN, SM ;

WOOLFORD, C ;

VANDERVIES, SM ;

TILLY, K ;

DENNIS, DT ;

GEORGOPOULOS, CP ;

HENDRIX, RW ;

ELLIS, RJ .

NATURE, 1988, 333 (6171) :330-334

[25] Chaperonin 60 phylogeny provides further evidence for secondary loss of mitochondria among putative early-branching eukaryotes [J].

Horner, DS ;

Embley, TM .

MOLECULAR BIOLOGY AND EVOLUTION, 2001, 18 (10) :1970-1975

[26] THE RAPID GENERATION OF MUTATION DATA MATRICES FROM PROTEIN SEQUENCES [J].

JONES, DT ;

TAYLOR, WR ;

THORNTON, JM .

COMPUTER APPLICATIONS IN THE BIOSCIENCES, 1992, 8 (03) :275-282

[27] Heat shock protein 60 sequence comparisons: Duplications, lateral transfer, and mitochondrial evolution [J].

Karlin, S ;

Brocchieri, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (21) :11348-11353

[28] Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi [J].

Katinka, MD ;

Duprat, S ;

Cornillot, E ;

Méténier, G ;

Thomarat, F ;

Prensier, G ;

Barbe, V ;

Peyretaillade, E ;

Brottier, P ;

Wincker, P ;

Delbac, F ;

El Alaoui, H ;

Peyret, P ;

Saurin, W ;

Gouy, M ;

Weissenbach, J ;

Vivarès, CP .

NATURE, 2001, 414 (6862) :450-453

[29] Polyamine biosynthesis in Cryptosporidium parvum and its implications for chemotherapy [J].

Keithly, JS ;

Zhu, G ;

Upton, SJ ;

Woods, KM ;

Martinez, MP ;

Yarlett, N .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 88 (1-2) :35-42

[30] EVALUATION OF THE MAXIMUM-LIKELIHOOD ESTIMATE OF THE EVOLUTIONARY TREE TOPOLOGIES FROM DNA-SEQUENCE DATA, AND THE BRANCHING ORDER IN HOMINOIDEA [J].

KISHINO, H ;

HASEGAWA, M .

JOURNAL OF MOLECULAR EVOLUTION, 1989, 29 (02) :170-179

← 1 2 3 4 5 6 →