Multiphoton multifocal microscopy exploiting a diffractive optical element

被引：131

作者：

Sacconi, L

Froner, E

Antolini, R

Taghizadeh, MR

Choudhury, A

Pavone, FS

机构：

[1] Univ Trent, I-38050 Trent, Italy

[2] Heriot Watt Univ, Dept Phys, Sch Engn & Phys Sci, Edinburgh EH14 4AS, Midlothian, Scotland

[3] European Lab Non Linear Spect, LENS, I-50019 Florence, Italy

[4] Univ Florence, Dept Phys, I-50019 Florence, Italy

[5] Ist Nazl Fis Mat, Sez Firenze, I-50019 Florence, Italy

来源：

OPTICS LETTERS | 2003年 / 28卷 / 20期

关键词：

D O I：

10.1364/OL.28.001918

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Multiphoton multifocal microscopy (MMM) usually has been achieved through a combination of galvo scanners with microlens arrays, with rotating disks of microlens arrays, and cascaded beam splitters with asynchronous rastering of scanning mirrors. Here we describe the achievement of a neat and compact MMM by use of a high-diffraction-efficiency diffractive-optic element that generates a multiple-spot grid of uniform intensity to achieve higher fidelity in imaging of live cells at adequate speeds. (C) 2003 Optical Society of America.

引用

页码：1918 / 1920

页数：3

共 10 条

[1] Multifocal multiphoton microscopy [J].