Kinetics of hepatitis B surface antigen-specific immune responses in acute and chronic hepatitis B or after HBs vaccination:: Stimulation of the in vitro antibody response by interferon gamma

被引:64
作者
Böcher, WO [1 ]
Herzog-Hauff, S [1 ]
Schlaak, J [1 ]
zum Büschenfelde, KHM [1 ]
Löhr, HF [1 ]
机构
[1] Univ Mainz, Dept Immunol, D-6500 Mainz, Germany
关键词
D O I
10.1002/hep.510290120
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Because cellular and humoral immune responses against the hepatitis B virus (HBV) surface antigen (HBs) might be crucial to overcome HBV infection, HBs-specific B- and T-cell responses of HBV patients and HBs vaccine recipients were analyzed quantitatively and functionally. In patients with acute hepatitis B (AHB), transient high anti-HBs-secreting B-cell frequencies were observed early after clinical onset, whereas 1 patient who probably developed chronic infection and chronic HBV carriers had absent or weak B- and T-cell responses. In HBs vaccine recipients, maximal HBs-specific B- and T-cell responses were detected after the first injection that decreased gradually before anti-HBs antibodies appeared in serum. Years after vaccination, anti-HBs-secreting B cells were enriched in the bone marrow. After in vitro stimulation with HBsAg, peripheral blood mononuclear cells (PBMC) of only 1 of 5 acute and 1 of 6 chronic HBV patients, but of all 6 vaccine recipients, secreted varying amounts of interferon gamma (IFN-gamma), but no interleukin-4 (IL-4) or IL-5. Furthermore, the addition of IFN-gamma, but not of IL-2, -4, -12, or IFN-alpha, resulted in strong increases of anti-HBs-secreting B cells in vaccine recipients and chronic carriers. In conclusion, circulating anti-HBs-secreting B cells were significantly higher in early acute hepatitis B or early after HBs vaccination than in chronic hepatitis B and decreased in the follow-up as a result of compartmentalization to lymphoid tissues. Release of IFN-gamma by antigen-stimulated T cells might be critical for anti-HBs formation.
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页码:238 / 244
页数:7
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共 36 条
  • [1] Th1-Th2: reliable paradigm or dangerous dogma?
    Allen, JE
    Maizels, RM
    [J]. IMMUNOLOGY TODAY, 1997, 18 (08): : 387 - 392
  • [2] BARNABA V, 1989, J IMMUNOL, V143, P2650
  • [3] Regulation of the neutralizing anti-hepatitis B surface (HBs) antibody response in vitro in HBs vaccine recipients and patients with acute or chronic hepatitis B virus (HBV) infection
    Bocher, WO
    HerzogHauff, S
    Herr, W
    Heermann, K
    Gerken, G
    zumBuschenfelde, KHM
    Lohr, HF
    [J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 1996, 105 (01) : 52 - 58
  • [4] IGG SUBCLASS DISTRIBUTION OF ANTI-HBS ANTIBODIES FOLLOWING VACCINATION WITH CDNA HBSAG
    BORZI, RM
    DALMONTE, P
    HONORATI, MC
    FACCHINI, A
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 146 (01) : 17 - 23
  • [5] DOHERTY PC, 1995, J IMMUNOL, V155, P1023
  • [6] IDENTIFICATION OF IMMUNODOMINANT T-CELL EPITOPES OF THE HEPATITIS-B VIRUS NUCLEOCAPSID ANTIGEN
    FERRARI, C
    BERTOLETTI, A
    PENNA, A
    CAVALLI, A
    VALLI, A
    MISSALE, G
    PILLI, M
    FOWLER, P
    GIUBERTI, T
    CHISARI, FV
    FIACCADORI, F
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (01) : 214 - 222
  • [7] FERRARI C, 1987, J IMMUNOL, V139, P2050
  • [8] FERRARI C, 1990, J IMMUNOL, V145, P3442
  • [9] MUTATION SPECIFIC PCR AND DIRECT SOLID-PHASE SEQUENCING ASSAY FOR THE DETECTION OF HEPATITIS-B VIRUS PRE-C/C MUTANTS IN ANTI-HBE-POSITIVE, CHRONIC HEPATITIS-B
    GOERGEN, B
    ZUMBUSCHENFELDE, KHM
    GERKEN, G
    [J]. JOURNAL OF MEDICAL VIROLOGY, 1994, 43 (01) : 97 - 102
  • [10] LARGE SURFACE-PROTEINS OF HEPATITIS-B VIRUS CONTAINING THE PRE-S SEQUENCE
    HEERMANN, KH
    GOLDMANN, U
    SCHWARTZ, W
    SEYFFARTH, T
    BAUMGARTEN, H
    GERLICH, WH
    [J]. JOURNAL OF VIROLOGY, 1984, 52 (02) : 396 - 402