Biocatalyst development by directed evolution

被引：102

作者：

Wang, Meng ^{[1
]}

Si, Tong ^{[1
]}

Zhao, Huimin ^{[1
,2
,3
,4
]}

机构：

[1] Univ Illinois, Dept Chem & Biomol Engn, Inst Genom Biol, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem, Inst Genom Biol, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Biochem, Inst Genom Biol, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Bioengn, Inst Genom Biol, Urbana, IL 61801 USA

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 115卷

基金：

美国国家科学基金会; 美国国家卫生研究院; 新加坡国家研究基金会;

关键词：

Directed evolution; Biocatalysis; Enzyme engineering; High-throughput screening; ITERATIVE SATURATION MUTAGENESIS; ENZYME EVOLUTION; ENANTIOSELECTIVE ENZYMES; DEHYDROGENASE-ACTIVITY; EPOXIDE HYDROLASE; SYNTHETIC BIOLOGY; TERTIARY ALCOHOLS; ESCHERICHIA-COLI; SCREENING SYSTEM; BIOMASS;

D O I：

10.1016/j.biortech.2012.01.054

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial applications. Recent advances in mutant library creation and high-throughput screening have greatly facilitated the engineering of novel and improved biocatalysts. This review provides an update of the recent developments in the use of directed evolution to engineer biocatalysts for practical applications. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：117 / 125

页数：9

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