Intestinal lactase-phlorizin hydrolase (LPH):: the two catalytic sites;: the role of the pancreas in pro-LPH maturation

被引：42

作者：

Zecca, L

Mesonero, JE

Stutz, A

Poirée, JC

Giudicelli, J

Cursio, R

Gloor, SM

Semenza, G ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, ETH Zentrum, Dept Biochem, CH-8092 Zurich, Switzerland

[2] Univ Milan, Dipartimento Chim & Biochim Med, Milan, Italy

[3] Univ Nice, Fac Med, Biochim Lab, F-06107 Nice, France

[4] Univ Nice, Fac Med, Chirurg Expt Lab, F-06107 Nice, France

来源：

FEBS LETTERS | 1998年 / 435卷 / 2-3期

关键词：

lactase; active site; proteolytic processing; pancreas;

D O I：

10.1016/S0014-5793(98)01076-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brush border lactase-phlorizin hydrolase carries two catalytic sites. In the human enzyme lactase comprises Glu-1749, phlorizin hydrolase Glu-1273. The proteolytic processing of pro-lactase-phlorizin hydrolase by (rat) enterocytes stops two amino acid residues short of the N-terminus of 'mature' final, brush border lactase-phlorizin hydrolase. Only these two amino acid residues are removed by luminal pancreatic protease(s), probably trypsin. (C) 1998 Federation of European Biochemical Societies.

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页码：225 / 228

页数：4

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