Generation of mouse-human hybridomas secreting antibodies against peanut allergen Ara h1

被引：2

作者：

Shinmoto, H

Naganawa, Y

Shimmoto, M

Maleki, SJ

机构：

[1] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[2] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1138613, Japan

[3] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA

来源：

CYTOTECHNOLOGY | 2004年 / 46卷 / 01期

关键词：

epitope; Epstein-Barr virus; human monoclonal antibody; human-mouse hybridoma; IgM; peanut allergen;

D O I：

10.1007/s10616-005-1578-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two clones of mouse-human hybridomas, secreting human monoclonal antibodies to a peanut allergen Ara h1, were generated from human peripheral blood lymphocytes transformed with Epstein-Barr virus, followed by cell fusion with mouse myeloma cells. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed antibody-binding sequences of Ara h1 protein.

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收藏

页码：19 / 23

页数：5

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