Preparation of human immortalized B-cells secreting antibodies against food allergens

被引：6

作者：

Shinmoto, H ^{[1
]}

Nakahara, K

Kobori, M

Tsushida, T

机构：

[1] Natl Food Res Inst, Tsukuba, Ibaraki 3058642 3058642, Japan

[2] Japan Int Res Ctr Agr Sci, Ibaraki, Osaka 3058686, Japan

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 07期

关键词：

D O I：

10.1023/A:1008807615539

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An immortalized B-cell library consisting of cells secreting antibodies to food allergens has been prepared. Peripheral B-cells from seven healthy donors were transformed with Epstein-Barr virus and an immortalized human B-cell library with 2,202 ampoules of multi-clone B-lymphoblastoid cells was obtained. The B-cell library contained various types of B-lymphoblastoid cells secreting antibodies to rice, soybeans, milk, and eggs, and can be used to analyze food allergens.

引用

页码：545 / 547

页数：3

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