Protein crystallization: from purified protein to diffraction-quality crystal

被引：270

作者：

Chayen, Naomi E. ^{[1
]}

Saridakis, Emmanuel ^{[2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Biomol Med, Div Surg Oncol Reprod Biol & Anaesthet, London SW7 2AZ, England

[2] Natl Ctr Sci Res Demokritos, Inst Phys Chem, Lab Struct & Supramol Chem, Athens 15310, Greece

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

基金：

英国工程与自然科学研究理事会;

关键词：

D O I：

10.1038/NMETH.F.203

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determining the structure of biological macromolecules by X-ray crystallography involves a series of steps: selection of the target molecule; cloning, expression, purification and crystallization; collection of diffraction data and determination of atomic positions. However, even when pure soluble protein is available, producing high-quality crystals remains a major bottleneck in structure determination. Here we present a guide for the non-expert to screen for appropriate crystallization conditions and optimize diffraction-quality crystal growth.

引用

收藏

页码：147 / 153

页数：7

相关论文

共 62 条

[11] Methods for separating nucleation and growth in protein crystallisation [J].

Chayen, NE .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2005, 88 (03) :329-337

[12] Turning protein crystallisation from an art into a science [J].

Chayen, NE .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2004, 14 (05) :577-583

[13] AN AUTOMATED-SYSTEM FOR MICROBATCH PROTEIN CRYSTALLIZATION AND SCREENING [J].

CHAYEN, NE ;

STEWART, PDS ;

MAEDER, DL ;

BLOW, DM .

JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1990, 23 :297-302

[14] Comparative studies of protein crystallization by vapour-diffusion and microbatch techniques [J].

Chayen, NE .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1998, 54 :8-15

[15] Trends and challenges in experimental macromolecular crystallography [J].

Chayen, NE ;

Boggon, TJ ;

Cassetta, A ;

Deacon, A ;

Gleichmann, T ;

Habash, J ;

Harrop, SJ ;

Helliwell, JR ;

Nieh, YP ;

Peterson, MR ;

Raftery, J ;

Snell, EH ;

Hadener, A ;

Niemann, AC ;

Siddons, DP ;

Stojanoff, V ;

Thompson, AW ;

Ursby, T ;

Wulff, M .

QUARTERLY REVIEWS OF BIOPHYSICS, 1996, 29 (03) :227-278

[16] Porous silicon: an effective nucleation-inducing material for protein crystallization [J].

Chayen, NE ;

Saridakis, E ;

El-Bahar, R ;

Nemirovsky, Y .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 312 (04) :591-595

[17] High-resolution crystal structure of an engineered human β2-adrenergic G protein-coupled receptor [J].

Cherezov, Vadim ;

Rosenbaum, Daniel M. ;

Hanson, Michael A. ;

Rasmussen, Soren G. F. ;

Thian, Foon Sun ;

Kobilka, Tong Sun ;

Choi, Hee-Jung ;

Kuhn, Peter ;

Weis, William I. ;

Kobilka, Brian K. ;

Stevens, Raymond C. .

SCIENCE, 2007, 318 (5854) :1258-1265

[18] Modified microbatch and seeding in protein crystallization experiments [J].

D'Arcy, A ;

Mac Sweeney, A ;

Haber, A .

JOURNAL OF SYNCHROTRON RADIATION, 2004, 11 :24-26

[19] CRYSTALLIZING PROTEINS - A RATIONAL APPROACH [J].

DARCY, A .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :469-471

[20] Protein crystallization: virtual screening and optimization [J].

DeLucas, LJ ;

Hamrick, D ;

Cosenza, L ;

Nagy, L ;

McCombs, D ;

Bray, T ;

Chait, A ;

Stoops, B ;

Belgovskiy, A ;

Wilson, WW ;

Parham, M ;

Chernov, N .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2005, 88 (03) :285-309

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