A phorbol ester-binding protein is required downstream of Rab5 is endosome fusion

被引:3
作者
Aballay, A
Barbieri, MA
Colombo, MI
Arenas, GN
Stahl, PD
Mayorga, LS
机构
[1] Univ Nacl Cuyo, CONICET, Fac Ciencias Med, Inst Histol & Embriol, RA-5500 Mendoza, Argentina
[2] Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
endosome fusion; endocytosis; protein kinase C; phorbol ester; Rab5;
D O I
10.1016/S0014-5793(98)01579-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous observations indicate that a zinc and phorbol ester binding factor is necessary for endosome fusion, To further characterize the role of this factor in the process, we used an in vitro endosome fusion assay supplemented with recombinant Rab5 proteins. Both zinc depletion and addition of calphostin C, an inhibitor of protein kinase C, inhibited endosome fusion in the presence of active Rab5. Addition of the phorbol ester PMA (phorbol 12-myristate 13-acetate) reversed the inhibition of endosome fusion caused by a Rab5 negative mutant. Moreover, PMA stimulated fusion in the presence of Rab5 immunodepleted cytosol. These results suggest that the phorbol ester binding protein is acting downstream of Rab5 in endosome fusion, (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:373 / 378
页数:6
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