Simple and convenient method for estimation of marker entrapped in liposomes

被引：13

作者：

Ishii, F ^{[1
]}

Nagasaka, Y ^{[1
]}

机构：

[1] Meiji Pharmaceut Univ, Dept Pharmaceut Sci, Tokyo 2048588, Japan

来源：

JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY | 2001年 / 22卷 / 01期

关键词：

liposome; calcein; ethanol; triton X-100; encapsulation efficiency;

D O I：

10.1081/DIS-100102684

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We have developed a new method to determine the marker calcein encapsulated in liposomes using ethanol or propanol instead of Triton X-100. Calcein in ethanol solution was estimated by fluorophotometry with excitation and emission wavelengths of 490 nm and 520 nm, respectively. The fluorescence intensity of calcein solubilized by ethanol was linear in the range of 10(-6)-10(-4) mol/L with a correlation coefficient of 0.999. The proposed method, which was accurate and reproducible, was successfully applied to determine the amounts of ethanol-soluble drugs in liposomes.

引用

页码：97 / 101

页数：5

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