A hot new twist to hair biology -: Involvement of vanilloid receptor-1 (VR1/TRPV1) signaling in human hair growth control

被引:157
作者
Bodó, E
Bíró, T
Telek, A
Czifra, G
Tóth, BI
Mescalchin, A
Ito, T
Bettermann, A
Kovács, L
Paus, R
机构
[1] Univ Debrecen, Dept Physiol, H-4012 Debrecen, Hungary
[2] Univ Debrecen, Cell Physiol Res Grp, Hungarian Acad Sci, Med & Hlth Sci Ctr,Res Ctr Mol Med, H-4012 Debrecen, Hungary
[3] Univ Hamburg, Hosp Eppendorf, Dept Dermatol, D-20246 Hamburg, Germany
[4] Cutech Srl, Venice, Italy
基金
匈牙利科学研究基金会;
关键词
D O I
10.1016/S0002-9440(10)62320-6
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The vanilloid receptor-1 (VR1, or transient receptor potential vanilloid-1 receptor, TRPV1) is activated by capsaicin, the key ingredient of hot peppers. TRPV1 was originally described on sensory neurons as a central integrator of various nociceptive stimuli. However, several human skin cell populations are also now recognized to express TRPV1, but with unknown function. Exploiting the human hair follicle (HF) as a prototypic epithelial-mesenchymal interaction system, we have characterized the BY expression of TRPV1 in situ and have examined TRPV1 signaling in organ-cultured human scalp BY and outer root sheath (ORS) keratinocytes in vitro. TRPV1 immunoreactivity was confined to distinct epithem compartments of the human HF, mainly to the ORS and hair matrix. in organ culture, TRPV1 activation by capsaicin resulted in a dose-dependent and TRPV1-specific inhibition of hair shaft elongation, suppression of proliferation, induction of apoptosis, premature RF regression (catagen), and up-regulation of intrafollicular transforming growth factor-beta(2). Cultured human ORS keratinocytes also expressed functional TRPV1, whose stimulation inhibited proliferation, induced apoptosis, elevated intraceltular calcium concentration, up-regulated known endogenous hair growth inhibitors (interleukin-1 beta, transforming growth factor-beta(2)), and down-regulated known hair growth promoters (hepatocyte growth factor, insulin-like growth factor-I, stem cell factor). These findings strongly support TRPV1 as a significant novel player in human hair growth control, underscore the physiological importance of TRPV1 in human skin beyond nociception, and identify TRPV1 as a promising, novel target for pharmacological manipulations of epithelial growth disorders.
引用
收藏
页码:985 / 998
页数:14
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