Deriving Excitatory Neurons of the Neocortex from Pluripotent Stem Cells

被引：80

作者：

Hansen, David V. ^{[1
,3
]}

Rubenstein, John L. R. ^{[2
,3
]}

Kriegstein, Arnold R. ^{[1
,3
]}

机构：

[1] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USA

来源：

NEURON | 2011年 / 70卷 / 04期

关键词：

RADIAL GLIAL-CELLS; OUTER SUBVENTRICULAR ZONE; SUBTYPE-SPECIFIC GENES; CEREBRAL-CORTEX; SELF-RENEWAL; PROGENITOR-CELL; LAMINAR FATE; IPS CELLS; IN-VITRO; HUMAN ES;

D O I：

10.1016/j.neuron.2011.05.006

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The human cerebral cortex is an immensely complex structure that subserves critical functions that can be disrupted in developmental and degenerative disorders. Recent innovations in cellular reprogramming and differentiation techniques have provided new ways to study the cellular components of the cerebral cortex. Here, we discuss approaches to generate specific subtypes of excitatory cortical neurons from pluripotent stem cells. We review spatial and temporal aspects of cortical neuron specification that can guide efforts to produce excitatory neuron subtypes with increased resolution. Finally, we discuss distinguishing features of human cortical development and their translational ramifications for cortical stem cell technologies.

引用

页码：645 / 660

页数：16

共 162 条

[1] Basic fibroblast growth factor endows dorsal telencephalic neural progenitors with the ability to differentiate into oligodendrocytes but not γ-aminobutyric acidergic neurons [J].