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FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16
被引:191
作者:

Heo, Kyu
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Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Kim, Hyunjung
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h-index: 0
机构:
Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Choi, Si Ho
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h-index: 0
机构:
Univ So Calif, Jane Anne Nohl Div Hematol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Choi, Jongkyu
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机构:
Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Kim, Kyunghwan
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Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Gu, Jiafeng
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机构:
Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
Univ So Calif, Dept Pathol, Los Angeles, CA 90089 USA
Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90089 USA
Univ So Calif, Norris Comprehens Canc Ctr, Dept Biol Sci, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Lieber, Michael R.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
Univ So Calif, Dept Pathol, Los Angeles, CA 90089 USA
Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90089 USA
Univ So Calif, Norris Comprehens Canc Ctr, Dept Biol Sci, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

Yang, Allen S.
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h-index: 0
机构:
Univ So Calif, Jane Anne Nohl Div Hematol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA

An, Woojin
论文数: 0 引用数: 0
h-index: 0
机构:
Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
机构:
[1] Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
[2] Univ So Calif, Jane Anne Nohl Div Hematol, Los Angeles, CA 90089 USA
[3] Univ So Calif, Dept Pathol, Los Angeles, CA 90089 USA
[4] Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90089 USA
[5] Univ So Calif, Norris Comprehens Canc Ctr, Dept Biol Sci, Los Angeles, CA 90089 USA
关键词:
D O I:
10.1016/j.molcel.2008.02.029
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The phosphorylation of histone variant H2AX at DNA double-strand breaks is believed to be critical for recognition and repair of DNA damage. However, little is known about the molecular mechanism regulating the exchange of variant H2AX with conventional H2A in the context of the nucleosome. Here, we isolate the H2AX-associated factors, which include FACT (Spt16/SSRP1), DNA-PK, and PARP1 from a human cell line. Our analyses demonstrate that the H2AX-associated factors efficiently promote both integration and dissociation of H2AX and this exchange reaction is mainly catalyzed by FACT among the purified factors. The phosphorylation of H2AX by DNA-PK facilitates the exchange of nucleosomal H2AX by inducing conformational changes of the nucleosome. In contrast, poly-ADP-ribosylation of Spt16 by PARP1 significantly inhibits FACT activities for H2AX exchange. Thus, these data establish FACT as the major regulator involved in H2AX exchange process that is modulated by H2AX phosphorylation and Spt16 ADP-ribosylation.
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页码:86 / 97
页数:12
相关论文
共 36 条
[11]
ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF
[J].
Hamiche, A
;
Sandaltzopoulos, R
;
Gdula, DA
;
Wu, C
.
CELL,
1999, 97 (07)
:833-842

Hamiche, A
论文数: 0 引用数: 0
h-index: 0
机构:
NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA

Sandaltzopoulos, R
论文数: 0 引用数: 0
h-index: 0
机构:
NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA

Gdula, DA
论文数: 0 引用数: 0
h-index: 0
机构:
NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA

Wu, C
论文数: 0 引用数: 0
h-index: 0
机构:
NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA
[12]
Isolation and characterization of proteins associated with histone H3 tails in vivo
[J].
Heo, Kyu
;
Kim, Bong
;
Kim, Kyunghwan
;
Choi, Jongkyu
;
Kim, Hyunjung
;
Zhan, Yuxia
;
Ranish, Jeffrey A.
;
An, Woojin
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2007, 282 (21)
:15476-15483

Heo, Kyu
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

Kim, Bong
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

Kim, Kyunghwan
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

Choi, Jongkyu
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

Kim, Hyunjung
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

Zhan, Yuxia
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

Ranish, Jeffrey A.
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA

An, Woojin
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Kenneth Norris Jr Comprehens Canc Ctr, Los Angeles, CA 90033 USA
[13]
Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation
[J].
Huang, Jing-Yi
;
Chen, Wei-Hao
;
Chang, Ya-Ling
;
Wang, Hsiao-Ting
;
Chuang, Wan-ting
;
Lee, Sheng-Chung
.
NUCLEIC ACIDS RESEARCH,
2006, 34 (08)
:2398-2407

Huang, Jing-Yi
论文数: 0 引用数: 0
h-index: 0
机构: Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan

Chen, Wei-Hao
论文数: 0 引用数: 0
h-index: 0
机构: Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan

Chang, Ya-Ling
论文数: 0 引用数: 0
h-index: 0
机构: Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan

Wang, Hsiao-Ting
论文数: 0 引用数: 0
h-index: 0
机构: Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan

Chuang, Wan-ting
论文数: 0 引用数: 0
h-index: 0
机构: Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan

Lee, Sheng-Chung
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan Natl Taiwan Univ, Coll Med, Inst Mol Med, Taipei 10764, Taiwan
[14]
SWI-SNF-mediated nucleosome remodeling: Role of histone octamer mobility in the persistence of the remodeled state
[J].
Jaskelioff, M
;
Gavin, IM
;
Peterson, CL
;
Logie, C
.
MOLECULAR AND CELLULAR BIOLOGY,
2000, 20 (09)
:3058-3068

Jaskelioff, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

Gavin, IM
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

Peterson, CL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

Logie, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA
[15]
NAD+-dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1
[J].
Kim, MY
;
Mauro, S
;
Gévry, N
;
Lis, JT
;
Kraus, WL
.
CELL,
2004, 119 (06)
:803-814

Kim, MY
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

Mauro, S
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

Gévry, N
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

Lis, JT
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

Kraus, WL
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA
[16]
A novel histone exchange factor, protein phosphatase 2Cγ mediates the exchange and dephosphorylation of H2A-H2B
[J].
Kimura, Hiroshi
;
Takizawa, Nanako
;
Allemand, Eric
;
Hori, Tetsuya
;
Iborra, Francisco J.
;
Nozaki, Naohito
;
Muraki, Michiko
;
Hagiwara, Masatoshi
;
Krainer, Adrian R.
;
Fukagawa, Tatsuo
;
Okawa, Katsuya
.
JOURNAL OF CELL BIOLOGY,
2006, 175 (03)
:389-400

Kimura, Hiroshi
论文数: 0 引用数: 0
h-index: 0
机构:
Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Takizawa, Nanako
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Allemand, Eric
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Hori, Tetsuya
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Iborra, Francisco J.
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Nozaki, Naohito
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Muraki, Michiko
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Hagiwara, Masatoshi
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Krainer, Adrian R.
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Fukagawa, Tatsuo
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan

Okawa, Katsuya
论文数: 0 引用数: 0
h-index: 0
机构: Kyoto Univ, Grad Sch Med, Horizontal Med Res Org, Nucl Funct & Dynam Unit,Sakyo Ku, Kyoto 6068501, Japan
[17]
Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions
[J].
Kusch, T
;
Florens, L
;
MacDonald, WH
;
Swanson, SK
;
Glaser, RL
;
Yates, JR
;
Abmayr, SM
;
Washburn, MP
;
Workman, JL
.
SCIENCE,
2004, 306 (5704)
:2084-2087

Kusch, T
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Florens, L
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

MacDonald, WH
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Swanson, SK
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Glaser, RL
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Yates, JR
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Abmayr, SM
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Washburn, MP
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA

Workman, JL
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Inst Med Res, Kansas City, MO 64110 USA
[18]
The role of chromatin during transcription
[J].
Li, Bing
;
Carey, Michael
;
Workman, Jerry L.
.
CELL,
2007, 128 (04)
:707-719

Li, Bing
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Med Res Inst, Kansas City, MO 64110 USA

Carey, Michael
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Med Res Inst, Kansas City, MO 64110 USA

Workman, Jerry L.
论文数: 0 引用数: 0
h-index: 0
机构: Stowers Med Res Inst, Kansas City, MO 64110 USA
[19]
Identification and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1
[J].
Li, BM
;
Navarro, S
;
Kasahara, N
;
Comai, L
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
2004, 279 (14)
:13659-13667

Li, BM
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA

Navarro, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA

Kasahara, N
论文数: 0 引用数: 0
h-index: 0
机构: Univ So Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA

Comai, L
论文数: 0 引用数: 0
h-index: 0
机构:
Univ So Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA Univ So Calif, Keck Sch Med, Dept Mol Microbiol & Immunol, Los Angeles, CA 90033 USA
[20]
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning
[J].
Lowary, PT
;
Widom, J
.
JOURNAL OF MOLECULAR BIOLOGY,
1998, 276 (01)
:19-42

Lowary, PT
论文数: 0 引用数: 0
h-index: 0
机构: Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

Widom, J
论文数: 0 引用数: 0
h-index: 0
机构: Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA