FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16

被引:191
作者
Heo, Kyu [1 ]
Kim, Hyunjung [1 ]
Choi, Si Ho [2 ]
Choi, Jongkyu [1 ]
Kim, Kyunghwan [1 ]
Gu, Jiafeng [1 ,3 ,4 ,5 ]
Lieber, Michael R. [1 ,3 ,4 ,5 ]
Yang, Allen S. [2 ]
An, Woojin [1 ]
机构
[1] Univ So Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
[2] Univ So Calif, Jane Anne Nohl Div Hematol, Los Angeles, CA 90089 USA
[3] Univ So Calif, Dept Pathol, Los Angeles, CA 90089 USA
[4] Univ So Calif, Dept Mol Microbiol & Immunol, Los Angeles, CA 90089 USA
[5] Univ So Calif, Norris Comprehens Canc Ctr, Dept Biol Sci, Los Angeles, CA 90089 USA
关键词
D O I
10.1016/j.molcel.2008.02.029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The phosphorylation of histone variant H2AX at DNA double-strand breaks is believed to be critical for recognition and repair of DNA damage. However, little is known about the molecular mechanism regulating the exchange of variant H2AX with conventional H2A in the context of the nucleosome. Here, we isolate the H2AX-associated factors, which include FACT (Spt16/SSRP1), DNA-PK, and PARP1 from a human cell line. Our analyses demonstrate that the H2AX-associated factors efficiently promote both integration and dissociation of H2AX and this exchange reaction is mainly catalyzed by FACT among the purified factors. The phosphorylation of H2AX by DNA-PK facilitates the exchange of nucleosomal H2AX by inducing conformational changes of the nucleosome. In contrast, poly-ADP-ribosylation of Spt16 by PARP1 significantly inhibits FACT activities for H2AX exchange. Thus, these data establish FACT as the major regulator involved in H2AX exchange process that is modulated by H2AX phosphorylation and Spt16 ADP-ribosylation.
引用
收藏
页码:86 / 97
页数:12
相关论文
共 36 条
[11]   ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF [J].
Hamiche, A ;
Sandaltzopoulos, R ;
Gdula, DA ;
Wu, C .
CELL, 1999, 97 (07) :833-842
[12]   Isolation and characterization of proteins associated with histone H3 tails in vivo [J].
Heo, Kyu ;
Kim, Bong ;
Kim, Kyunghwan ;
Choi, Jongkyu ;
Kim, Hyunjung ;
Zhan, Yuxia ;
Ranish, Jeffrey A. ;
An, Woojin .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (21) :15476-15483
[13]   Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation [J].
Huang, Jing-Yi ;
Chen, Wei-Hao ;
Chang, Ya-Ling ;
Wang, Hsiao-Ting ;
Chuang, Wan-ting ;
Lee, Sheng-Chung .
NUCLEIC ACIDS RESEARCH, 2006, 34 (08) :2398-2407
[14]   SWI-SNF-mediated nucleosome remodeling: Role of histone octamer mobility in the persistence of the remodeled state [J].
Jaskelioff, M ;
Gavin, IM ;
Peterson, CL ;
Logie, C .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (09) :3058-3068
[15]   NAD+-dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1 [J].
Kim, MY ;
Mauro, S ;
Gévry, N ;
Lis, JT ;
Kraus, WL .
CELL, 2004, 119 (06) :803-814
[16]   A novel histone exchange factor, protein phosphatase 2Cγ mediates the exchange and dephosphorylation of H2A-H2B [J].
Kimura, Hiroshi ;
Takizawa, Nanako ;
Allemand, Eric ;
Hori, Tetsuya ;
Iborra, Francisco J. ;
Nozaki, Naohito ;
Muraki, Michiko ;
Hagiwara, Masatoshi ;
Krainer, Adrian R. ;
Fukagawa, Tatsuo ;
Okawa, Katsuya .
JOURNAL OF CELL BIOLOGY, 2006, 175 (03) :389-400
[17]   Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions [J].
Kusch, T ;
Florens, L ;
MacDonald, WH ;
Swanson, SK ;
Glaser, RL ;
Yates, JR ;
Abmayr, SM ;
Washburn, MP ;
Workman, JL .
SCIENCE, 2004, 306 (5704) :2084-2087
[18]   The role of chromatin during transcription [J].
Li, Bing ;
Carey, Michael ;
Workman, Jerry L. .
CELL, 2007, 128 (04) :707-719
[19]   Identification and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1 [J].
Li, BM ;
Navarro, S ;
Kasahara, N ;
Comai, L .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (14) :13659-13667
[20]   New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning [J].
Lowary, PT ;
Widom, J .
JOURNAL OF MOLECULAR BIOLOGY, 1998, 276 (01) :19-42