Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs

被引：66

作者：

Lange, TS

Borovjagin, A

Maxwell, ES

Gerbi, SA ^{[1
]}

机构：

[1] Brown Univ, Div Biol & Med, Providence, RI 02912 USA

[2] N Carolina State Univ, Dept Biochem, Raleigh, NC 27695 USA

来源：

EMBO JOURNAL | 1998年 / 17卷 / 11期

关键词：

Boxes C and D; nucleolar localization; snoRNA;

D O I：

10.1093/emboj/17.11.3176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequences necessary for nucleolar targeting were identified in Box C/D small nucleolar RNAs (snoRNAs) by fluorescence microscopy. Nucleolar preparations were examined after injecting fluorescein-labelled wildtype and mutated U14 or U8 snoRNA into Xenopus oocyte nuclei. Regions in U14 snoRNA that are complementary to 18S rRNA and necessary for rRNA processing and methylation are nob required for nucleolar localization. Truncated U14 molecules containing Boxes C and D with or without the terminal stem localized efficiently. Nucleolar localization was abolished upon mutating just one or two nucleotides within Boxes C and D. Moreover, the spatial position of Boxes C or D in the molecule is essential. Mutations in Box C/D of U8 snoRNA also impaired nucleolar localization, suggesting the general importance of Boxes C and D as nucleolar localization sequences for Box C/D snoRNAs. U14 snoRNA is shown to be required for 18S rRNA production in vertebrates.

引用

页码：3176 / 3187

页数：12

共 77 条

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