Microfluidics for flow cytometric analysis of cells and particles

被引：323

作者：

Huh, D ^{[1
]}

Gu, W

Kamotani, Y

Grotberg, JB

Takayama, S

机构：

[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Macromol Sci & Engn, Ann Arbor, MI 48109 USA

来源：

PHYSIOLOGICAL MEASUREMENT | 2005年 / 26卷 / 03期

关键词：

flow cytometry; microfluidics; microfabrication; cell analysis; cell separation;

D O I：

10.1088/0967-3334/26/3/R02

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

This review describes recent developments in microfabricated flow cytometers and related microfluidic devices that can detect, analyze, and sort cells or particles. The high-speed analytical capabilities of flow cytometry depend on the cooperative use of microfluidics, optics and electronics. Along with the improvement of other components, replacement of conventional glass capillary-based fluidics with microfluidic sample handling systems operating in microfabricated structures enables volume- and power-efficient, inexpensive and flexible analysis of particulate samples. In this review, we present various efforts that take advantage of novel microscale flow phenomena and microfabrication techniques to build microfluidic cell analysis systems.

引用

页码：R73 / R98

页数：26

共 76 条

[1]

Altendorf E, 1997, TRANSDUCERS 97 - 1997 INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS AND ACTUATORS, DIGEST OF TECHNICAL PAPERS, VOLS 1 AND 2, P531, DOI 10.1109/SENSOR.1997.613704

[2]

Arai F, 2001, ELECTROPHORESIS, V22, P283, DOI 10.1002/1522-2683(200101)22:2<283::AID-ELPS283>3.0.CO

[3]

2-C

[4] Current status of flow cytometry in cell and molecular biology [J].

Boeck, G .

INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 204, 2001, 204 :239-298

[5]

CABUZ E, 2002, P SOL STAT SENS ACT, P110

[6] Cytometric analysis of protein expression and apoptosis in human primary cells with a novel microfluidic chip-based system [J].

Chan, SDH ;

Luedke, G ;

Valer, M ;

Buhlmann, C ;

Preckel, T .

CYTOMETRY PART A, 2003, 55A (02) :119-125

[7] Microfabricated silicon flow-cell for optical monitoring of biological fluids [J].

Chau, LK ;

Osborn, T ;

Wu, CC ;

Yager, P .

ANALYTICAL SCIENCES, 1999, 15 (08) :721-724

[8] Passively driven integrated microfluidic system for separation of motile sperm [J].

Cho, BS ;

Schuster, TG ;

Zhu, XY ;

Chang, D ;

Smith, GD ;

Takayama, S .

ANALYTICAL CHEMISTRY, 2003, 75 (07) :1671-1675

[9]

Chung S, 2003, MICROSYST TECHNOL, V9, P525, DOI [10.1007/s00542-003-0302-2, 10.1007/S00542-003-0302-2]

[10] CORRELATED MEASUREMENTS OF DNA, RNA, AND PROTEIN IN INDIVIDUAL CELLS BY FLOW-CYTOMETRY [J].

CRISSMAN, HA ;

DARZYNKIEWICZ, Z ;

TOBEY, RA ;

STEINKAMP, JA .

SCIENCE, 1985, 228 (4705) :1321-1324

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