Analysis of Stevia glycosides by capillary electrophoresis

被引：34

作者：

Mauri, P

Catalano, G

Gardana, C

Pietta, P

机构：

[1] Ist. Technologie Biomediche Avanzate, Segrate (Milano)

[2] Ist. Tecnologie Biochmediche A., C/o LITA, 20096 Segrate (Milano)

来源：

ELECTROPHORESIS | 1996年 / 17卷 / 02期

关键词：

micellar electrokinetic capillary chromatography; Stevia rebaudinana; Stevioside;

D O I：

10.1002/elps.1150170213

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of diterpene glycosides from Stevia rebaudiana leaves using capillary electrophoresis is described. Analyses were performed on fused silica capillaries with 20 mM sodium tetraborate buffer, pH 8.3, and 30 mM sodium dodecyl sulfate. The effect of the organic solvent injected with the sample solution on the electrophoretic solution has been confirmed, and an absolute amount of 1.6 nL per injected sample was optimal. Rebaudioside A and steviolbioside were isolated by semipreparative high performance liquid chromatography (HPLC), and their structure was assessed by mass spectrometry.

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收藏

页码：367 / 371

页数：5

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