Noninvasive measurement of concurrent single-kidney perfusion, glomerular filtration, and tubular function

被引:134
作者
Krier, JD
Ritman, EL
Bajzer, Z
Romero, JC
Lerman, A
Lerman, LO
机构
[1] Mayo Clin & Mayo Fdn, Dept Internal Med, Div Hypertens, Rochester, MN 55905 USA
[2] Mayo Clin & Mayo Fdn, Dept Internal Med, Div Cardiovasc Dis, Rochester, MN 55905 USA
[3] Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[4] Mayo Clin & Mayo Fdn, Dept Biochem & Mol Biol, Rochester, MN 55905 USA
关键词
renal blood flow; glomerular filtration rate; electron beam computed tomography;
D O I
10.1152/ajprenal.2001.281.4.F630
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To assess the reliability of electron beam computed tomography (EBCT), measurements of single-kidney renal blood flow (RBF), glomerular filtration rate (GFR), and intratubular contrast medium concentration (ITC) of radiographic contrast media were quantified in anesthetized pigs before and after acetylcholine-induced vasodilation and diuresis. EBCT measurements were compared with those obtained with intravascular Doppler and inulin clearance. The capability of EBCT to detect chronic changes in single-kidney function was evaluated in pigs with unilateral renal artery stenosis, and their long-term reproducibility in normal pigs was studied repeatedly at 1-mo intervals. EBCT-RBF (ml/min) correlated with Doppler-RBF as RBF(EBCT) = 45 + 1.07 (*) RBF(Doppler), r = 0.81. EBCT-GFR (ml/min) correlated with inulin clearance as GFR(EBCT) = 11.7 + 1.02 (*) GFR(inulin), r = 0.80. During vasodilation, RBF and GFR increased, whereas ITC decreased along the nephron. In renal artery stenosis, single-kidney GFR decreased linearly with the degree of stenosis, and ITC increased along the nephron, indicating increased fluid reabsorption. EBCT-RBF, GFR, and ITC were similar among repeated measurements. This approach might be invaluable for simultaneous quantification of regional hemodynamics and function in the intact kidneys, in a manner potentially applicable to humans.
引用
收藏
页码:F630 / F638
页数:9
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