Computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality

被引：121

作者：

Robinson, Christopher M. ^{[1
,5
]}

Singh, Gurdeep ^{[1
]}

Henquell, Cecile ^{[2
]}

Walsh, Michael P. ^{[3
]}

Peigue-Lafeuille, Helene ^{[2
]}

Seto, Donald ^{[3
]}

Jones, Morris S. ^{[4
]}

Dyer, David W. ^{[5
]}

Chodosh, James ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Eye & Ear Infirm, Sch Med, Howe Lab,Dept Ophthalmol, Boston, MA 02114 USA

[2] Univ Auvergne, Hop Gabriel Montpied, Virol Lab, CHU Clermont Ferrand, Clermont Ferrand, France

[3] George Mason Univ, Dept Bioinformat & Computat Biol, Manassas, VA 20110 USA

[4] David Grant USAF Med Ctr, Clin Invest Facil, Fairfield, CA 94535 USA

[5] Univ Oklahoma, Hlth Sci Ctr, Dept Microbiol & Immunol, Oklahoma City, OK 73104 USA

来源：

VIROLOGY | 2011年 / 409卷 / 02期

关键词：

Adenovirus; Viral genomics; Viral recombination; Viral evolution; Viral bioinformatics; SIALIC-ACID; EPIDEMIC KERATOCONJUNCTIVITIS; FIBER KNOBS; PROTEIN; INFECTION; RECEPTOR; DISEASE; BINDING; SEQUENCE; VIRUS;

D O I：

10.1016/j.virol.2010.10.020

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Adenoviral infections are typically acute, self-limiting, and not associated with death. However, we present the genomic and bioinformatics analysis of a novel recombinant human adenovirus (HAdV-D56) isolated in France that caused a rare neonatal fatality, and keratoconjunctivitis in three health care workers who cared for the neonate. Whole genome alignments revealed the expected diversity in the penton base, hexon, E3, and fiber coding regions, and provided evidence for extensive recombination. Bootscan analysis confirmed recombination between HAdV-D9, HAdV-D26, HAdV-D15, and HAdV-D29 in the penton base and hexon proteins, centered around hypervariable loops within the putative proteins. Protein structure analysis of the fiber coding region revealed similarity with HAdV-D8, HAdV-D9, and HAdV-D53, possibly accounting for the ocular tropism of the virus. Based on these data, this virus appears to be a new HAdV-D type (HAdV-D56), underscoring the importance of recombination events in human adenovirus evolution and the emergence of new adenovirus pathogens. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：141 / 147

页数：7

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