Immobilization of urease in conducting thiophene-capped poly(methyl methacrylate)/pyrrole matrices

被引：16

作者：

Alkan, S

Toppare, L ^{[1
]}

Bakir, U

Yagci, Y

机构：

[1] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

[2] Middle E Tech Univ, Dept Food Engn, TR-06531 Ankara, Turkey

[3] Istanbul Tech Univ, Dept Chem, TR-80626 Istanbul, Turkey

来源：

SYNTHETIC METALS | 2001年 / 123卷 / 01期

关键词：

immobilization; urease; polypyrrole; poly(methyl methacrylate); electropolymerization;

D O I：

10.1016/S0379-6779(00)00580-4

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Urease was immobilized in conducting polypyrrole and block copolymers of thiophene-capped poly(methyl methacrylate) matrices by electropolymerization. Immobilization of the enzyme was achieved by application of 1.0 V constant potential in a solution of 0.02 M pyrrole, 2 mg/ml urease, 0.5 mg/ml supporting electrolyte. The optimum immobilization conditions, electrolysis time, electrolysis medium, enzyme concentration during electrolysis, type of supporting electrolyte were determined. The kinetic behaviors of free and immobilized urease were determined. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：95 / 99

页数：5

共 23 条

[1]

Alkan S, 1999, J POLYM SCI POL CHEM, V37, P4218, DOI 10.1002/(SICI)1099-0518(19991115)37:22<4218::AID-POLA22>3.0.CO

[2]

2-Z

[3] Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices [J].