Permeabilization of Pichia pastoris for glycolate oxidase activity

被引：13

作者：

Gough, S ^{[1
]}

Deshpande, M ^{[1
]}

Scher, M ^{[1
]}

Rosazza, JPN ^{[1
]}

机构：

[1] Univ Iowa, Ctr Biocatalysis & Bioproc, Iowa City, IA 52242 USA

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 18期

关键词：

glycolate oxidase; permeabilization; Pichia pastoris; yeast;

D O I：

10.1023/A:1011601827503

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Maximum activity of glycolate oxidase was obtained from a recombinant Pichia pastoris by permeabilization with 0.1% benzalkonium chloride for 60 min at room temperature. After treatment, intracellular glycolate oxidase activity increased 10-fold with respect to untreated cells.

引用

页码：1535 / 1537

页数：3

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