PERMEABILIZATION OF Kluyveromyces lactis CELLS FOR MILK WHEY SACCHARIFICATION: A COMPARISON OF DIFFERENT TREATMENTS

被引：27

作者：

Siso, M. I. G. ^{[1
]}

Cerdan, E. ^{[1
]}

Picos, M. A. F. ^{[1
]}

Ramil, E. ^{[1
]}

Belmonte, E. R. ^{[1
]}

Torres, A. R. ^{[1
]}

机构：

[1] Univ A Coruna, Dept Biol Celular & Mol, La Coruna, Spain

来源：

BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 04期

关键词：

D O I：

10.1007/BF02439313

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we compare the effectiveness of various lactose permeabilizatlon treatments for Kluyveromyces lactis. Best yields are obtained with 4: 96 toluene: ethanol and with 70% ethanol in mild conditions, expression of intracellular beta-galectosldese activity being Increased 40-fold with respect to untreated cells. Permeabillzed cells hydrolyse lactose from milk whey more rapidly than control cells end do not further metabolize monosaccharides. This is a desired characteristic in terms of their potential commercial application.

引用

页码：289 / 292

页数：4

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