BMPs functionally replace Klf4 and support efficient reprogramming of mouse fibroblasts by Oct4 alone

被引：104

作者：

Chen, Jiekai ^{[1
]}

Liu, Jing ^{[1
]}

Yang, Jiaqi ^{[1
]}

Chen, You ^{[1
]}

Chen, Jing ^{[1
]}

Ni, Su ^{[1
]}

Song, Hong ^{[1
]}

Zeng, Lingwen ^{[1
]}

Ding, Ke ^{[1
]}

Pei, Duanqing ^{[1
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, S China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou 510530, Guangdong, Peoples R China

来源：

CELL RESEARCH | 2011年 / 21卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Oct4; iPSCs; reprogramming; stem cell; BMPs; MET; PLURIPOTENT STEM-CELLS; GENERATION; INDUCTION; MYC;

D O I：

10.1038/cr.2010.172

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Generation of induced pluripotent stem cells by defined factors has become a useful model to investigate the mechanism of reprogramming and cell fate determination. However, the precise mechanism of factor-based reprogramming remains unclear. Here, we show that Klf4 mainly acts at the initial phase of reprogramming to initiate mesenchymal-to-epithelial transition and can be functionally replaced by bone morphogenetic proteins (BMPs). BMPs boosted the efficiency of Oct4/Sox2-mediated reprogramming of mouse embryonic fibroblasts (MEFs) to similar to 1%. BMPs also promoted single-factor Oct4-based reprogramming of MEFs and tail tibial fibroblasts. Our studies clarify the contribution of Klf4 in reprogramming and establish Oct4 as a singular setter of pluripotency in differentiated cells.

引用

页码：205 / 212

页数：8

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