Towards an Optimized Culture Medium for the Generation of Mouse Induced Pluripotent Stem Cells

被引：51

作者：

Chen, Jiekai ^{[1
]}

Liu, Jing ^{[1
]}

Han, Qingkai ^{[1
]}

Qin, Dajiang ^{[1
]}

Xu, Jianyong ^{[1
]}

Chen, You ^{[1
]}

Yang, Jiaqi ^{[1
]}

Song, Hong ^{[1
]}

Yang, Dongshan ^{[1
]}

Peng, Meixiu ^{[1
]}

He, Wenzhi ^{[1
]}

Li, Ronghui ^{[1
]}

Wang, Hao ^{[1
]}

Gan, Yi ^{[1
]}

Ding, Ke ^{[1
]}

Zeng, Lingwen ^{[1
]}

Lai, Liangxue ^{[1
]}

Esteban, Miguel A. ^{[1
]}

Pei, Duanqing ^{[1
]}

机构：

[1] Chinese Acad Sci, Stem Cell & Canc Biol Grp, Chinese Acad Sci Key Lab Regenerat Biol, S China Inst Stem Cell Biol & Regenerat Med,Guang, Guangzhou 510530, Guangdong, Peoples R China

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 40期

基金：

中国国家自然科学基金;

关键词：

EMBRYONIC FIBROBLASTS; INDUCTION; PROMOTE;

D O I：

10.1074/jbc.M110.139436

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Generation of induced pluripotent stem cells from somatic cells using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient and time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation of mouse induced pluripotent stem cells. This optimization of the culture medium is sensitive to the presence of Myc in the reprogramming factors. Moreover, we could reprogram meningeal cells using only two factors Oct4/Klf4. Therefore, iSF1 represents a basal medium that may be used for mechanistic studies and testing new reprogramming approaches.

引用

收藏

页码：31066 / 31072

页数：7

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