Synthesis and properties of oligonucleotides containing 8-bromo-2′-deoxyguanosine

被引：13

作者：

Fàbrega, C ^{[1
]}

Macías, MJ ^{[1
]}

Eritja, R ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 03期

关键词：

D O I：

10.1081/NCN-100002085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of oligonucleotides containing 8-bromo-2 ' -deoxyguanosine is described. Substitution of G by 8-bromoguanine on an alternating CG decamer stabilizes the Z-form in such a way that the B-form was not observed. Melting temperatures showed that duplexes in which 8-bromo-2 ' -deoxyguanosine paired with natural bases were much less stable.

引用

页码：251 / 260

页数：10

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