Studies on the synthesis of oligonucleotides containing photoreactive nucleosides:: 2-azido-2′-deoxyinosine and 8-azido-2′-deoxyadenosine

被引：5

作者：

Fàbrega, C ^{[1
]}

García, RG ^{[1
]}

Díaz, AR ^{[1
]}

Eritja, R ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

BIOLOGICAL CHEMISTRY | 1998年 / 379卷 / 4-5期

关键词：

8-azido-2 '-deoxyadenosine; 2-azido-2 '-deoxyinosine; azidonucleosides; oligonucleotides; photocrosslinking; photoreactive nucleosides;

D O I：

10.1515/bchm.1998.379.4-5.527

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligonucleotides containing the photoreactive nucleosides 2-azido-2'-deoxyinosine and 8-azido-2'-deoxyadenosine have been prepared using protected 2-fluoro-2'-deoxyinosine and 8-bromo-2'-deoxyadenosine phosphoramidites. After the assembly of the oligonucleotides, the nucleoside derivatives are converted to the corresponding azido derivatives by treatment with lithium azide in dry DMF. Deprotection of oligonucleotides carrying these azidonucleosides is performed with concentrated ammonia at room temperature.

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收藏

页码：527 / 533

页数：7

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