Synthesis of oligodeoxynucleotides containing 2-substituted guanine derivatives using 2-fluoro-2'-deoxyinosine as common nucleoside precursor.

被引：21

作者：

Diaz, AR ^{[1
]}

Eritja, R ^{[1
]}

Garcia, RG ^{[1
]}

机构：

[1] EUROPEAN MOL BIOL LAB,D-69117 HEIDELBERG,GERMANY

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 10-11期

关键词：

D O I：

10.1080/07328319708002554

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Oligonucleotides containing 2-substituted guanine derivatives with double-helix stabilizing molecules such as spermine, spermidine and propylimidazole have been prepared using protected 2-fluoro-2'-deoxyinosine phosphoramidite and two different protective strategies: the p-nitrophenylethyl (NPE) and the t-butylphenoxyacetyl groups. Melting studies show a large increase on the melting temperatures of duplexes containing these 2-substituted guanine derivatives.

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页码：2035 / 2051

页数：17

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