A comparative study of supports for the synthesis of oligonucleotides without using ammonia

被引：19

作者：

Avino, A

Garcia, RG

Diaz, A

Albericio, F

Eritja, R

机构：

[1] CSIC,CID,ES-08034 BARCELONA,SPAIN

[2] EUROPEAN MOL BIOL LAB,D-69117 HEIDELBERG,GERMANY

[3] UNIV BARCELONA,DPT QUIM ORGAN,E-08028 BARCELONA,SPAIN

来源：

NUCLEOSIDES & NUCLEOTIDES | 1996年 / 15卷 / 11-12期

关键词：

D O I：

10.1080/07328319608002739

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparative study of the cleavage efficiency of succinyl, phthaloyl, oxalyl, 2-(2-nitrophenyl)ethyl, 9-fluorenylmethyl, and 2-nitrobenzyl supports in 0.5M DBU solutions is described. A decrease in cleavage efficiency is observed when small oligonucleotides containing thymidine are linked to the supports. In these conditions oxalyl supports gave the best yields followed by 2-(2-nitrophenyl)ethyl and 8-fluorenylmethyl supports.

引用

页码：1871 / 1889

页数：19

共 26 条

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