Preparation and properties of oligodeoxynucleotides containing 5-iodouracil and 5-bromo- and 5-iodocytosine

被引：21

作者：

Ferrer, E

Wiersma, M

Kazimierczak, B

Muller, CW

Eritja, R

机构：

[1] EUROPEAN MOL BIOL LAB,D-69117 HEIDELBERG,GERMANY

[2] ILL GRENOBLE,GRENOBLE OUTSTN,EUROPEAN MOL BIOL LAB,F-38042 GRENOBLE 9,FRANCE

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 05期

关键词：

D O I：

10.1021/bc970042l

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The behavior of oligonucleotides containing 5-iodouracil, 5-bromocytidine, and 5-iodocytidine in concentrated ammonia is described. 5-Aminouracil and 5-aminocytidine are obtained as side products when deprotection is performed at 60 degrees C. Small amounts, if any, of side products are obtained when ammonia deprotection is performed at room temperature. The base-pairing properties of these 5-halopyrimidines including triple helix are described.

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页码：757 / 761

页数：5

相关论文

共 29 条

[1]

DICKERSON RE, 1992, METHOD ENZYMOL, V211, P67

[2] IDENTIFICATION OF AMINO-ACID BASE CONTACTS IN THE MYC DNA COMPLEX BY SITE-SPECIFIC BROMOURACIL MEDIATED PHOTO-CROSS-LINKING [J].

DONG, QP ;

BLATTER, EE ;

EBRIGHT, YW ;

BISTER, K ;

EBRIGHT, RH .

EMBO JOURNAL, 1994, 13 (01) :200-204

[3] Preparation of oligonucleotides containing 5-bromouracil and 5-methylcytidine. [J].

Ferrer, E ;

Fabrega, C ;

Garcia, RG ;

Azorin, F ;

Eritja, R .

NUCLEOSIDES & NUCLEOTIDES, 1996, 15 (04) :907-921

[4]

FERRER E, 1997, IN PRESS J CHEM S P1

[5] ANALYSIS OF THE RECOGNITION MECHANISM INVOLVED IN THE ECORV CATALYZED CLEAVAGE OF DNA USING MODIFIED OLIGODEOXYNUCLEOTIDES [J].

FLIESS, A ;

WOLFES, H ;

SEELA, F ;

PINGOUD, A .

NUCLEIC ACIDS RESEARCH, 1988, 16 (24) :11781-11793

[6] BINDING OF THE ECORII METHYLTRANSFERASE TO 5-FLUOROCYTOSINE-CONTAINING DNA - ISOLATION OF A BOUND PEPTIDE [J].

FRIEDMAN, S ;

ANSARI, N .

NUCLEIC ACIDS RESEARCH, 1992, 20 (12) :3241-3248

[7]

GOLMAN D, 1983, NUCLEOS NUCLEOT, V2, P175

[8] 5-FLUORODEOXYURIDINE AS AN ALTERNATIVE TO THE SYNTHESIS OF MIXED HYBRIDIZATION PROBES FOR THE DETECTION OF SPECIFIC GENE-SEQUENCES [J].

HABENER, JF ;

VO, CD ;

LE, DB ;

GRYAN, GP ;

ERCOLANI, L ;

WANG, AHJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) :1735-1739

[9] SEQUENCE-SPECIFIC AND MECHANISM-BASED CROSS-LINKING OF DCM DNA CYTOSINE-C5 METHYLTRANSFERASE OF ESCHERICHIA-COLI K-12 TO SYNTHETIC OLIGONUCLEOTIDES CONTAINING 5-FLUORO-2'-DEOXYCYTIDINE [J].

HANCK, T ;

SCHMIDT, S ;

FRITZ, HJ .

NUCLEIC ACIDS RESEARCH, 1993, 21 (02) :303-309

[10] SYNTHESIS OF DECADEOXYRIBONUCLEOTIDES CONTAINING 5-MODIFIED URACILS AND THEIR INTERACTIONS WITH RESTRICTION ENDONUCLEASES BGL II, SAU 3AI AND MBO-I (NUCLEOSIDES AND NUCLEOTIDES .82. [J].

HAYAKAWA, T ;

ONO, A ;

UEDA, T .

NUCLEIC ACIDS RESEARCH, 1988, 16 (11) :4761-4776