A gene for a Class II DNA photolyase from Oryza sativa:: cloning of the cDNA by dilution-amplification

被引:35
作者
Hirouchi, T
Nakajima, S
Najrana, T
Tanaka, M
Matsunaga, T
Hidema, J
Teranishi, M
Fujino, T
Kumagai, T
Yamamoto, K [1 ]
机构
[1] Tohoku Univ, Grad Sch Life Sci, Dept Mol Biosci, Sendai, Miyagi 9808577, Japan
[2] Kanazawa Univ, Fac Pharmaceut Sci, Lab Mol Human Genet, Kanazawa, Ishikawa 9200934, Japan
[3] Tohoku Univ, Grad Sch Life Sci, Dept Environm Life Sci, Sendai, Miyagi 9808577, Japan
基金
日本学术振兴会;
关键词
photolyase; Oryza sativa; solar UV radiation; alternative splicing; GC content;
D O I
10.1007/s00438-003-0856-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ultraviolet radiation induces the formation of two classes of photoproducts in DNA-the cyclobutane pyrimidine dimer (CPD) and the pyrimidine [6-4] pyrimidone photoproduct (6-4 product). Many organisms produce enzymes, termed photolyases, which specifically bind to these lesions and split them via a UV-A/blue light-dependent mechanism, thereby reversing the damage. These photolyases are specific for either CPDs or 6-4 products. Two classes of photolyases (class I and class 11) repair CPDs. A gene that encodes a protein with class 11 CPD photolyase activity in vitro has been cloned from several plants including Arabidopsis thaliana, Cucumis sativus and Chlamydomonas reinhardtii. We report here the isolation of a homolog of this gene from rice (Oryza sativa), which was cloned on the basis of sequence similarity and PCR-based dilution-amplification. The cDNA comprises a very GC-rich (75%) 5' region, while the 3' portion has a GC content of 50%. This gene encodes a protein with CPD photolyase activity when expressed in E. coli. The CPD photolyase gene encodes at least two types of mRNA, formed by alternative splicing of exon 5. One of the mRNAs encodes an ORF for 506 amino acid residues, while the other is predicted to code for 364 amino acid residues. The two RNAs occur in about equal amounts in O. sativa cells.
引用
收藏
页码:508 / 516
页数:9
相关论文
共 39 条
[1]   An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis [J].
Ahmad, M ;
Jarillo, JA ;
Klimczak, LJ ;
Landry, LG ;
Peng, T ;
Last, RL ;
Cashmore, AR .
PLANT CELL, 1997, 9 (02) :199-207
[2]   HY4 GENE OF A-THALIANA ENCODES A PROTEIN WITH CHARACTERISTICS OF A BLUE-LIGHT PHOTORECEPTOR [J].
AHMAD, M ;
CASHMORE, AR .
NATURE, 1993, 366 (6451) :162-166
[3]   Molecular genetics of DNA repair in higher plants [J].
Britt, AB .
TRENDS IN PLANT SCIENCE, 1999, 4 (01) :20-25
[4]   REPAIR OF DNA-DAMAGE INDUCED BY ULTRAVIOLET-RADIATION [J].
BRITT, AB .
PLANT PHYSIOLOGY, 1995, 108 (03) :891-896
[5]   Compositional properties of homologous coding sequences from plants [J].
Carels, N ;
Hatey, P ;
Jabbari, K ;
Bernardi, G .
JOURNAL OF MOLECULAR EVOLUTION, 1998, 46 (01) :45-53
[6]   A draft sequence of the rice genome (Oryza sativa L. ssp japonica) [J].
Goff, SA ;
Ricke, D ;
Lan, TH ;
Presting, G ;
Wang, RL ;
Dunn, M ;
Glazebrook, J ;
Sessions, A ;
Oeller, P ;
Varma, H ;
Hadley, D ;
Hutchinson, D ;
Martin, C ;
Katagiri, F ;
Lange, BM ;
Moughamer, T ;
Xia, Y ;
Budworth, P ;
Zhong, JP ;
Miguel, T ;
Paszkowski, U ;
Zhang, SP ;
Colbert, M ;
Sun, WL ;
Chen, LL ;
Cooper, B ;
Park, S ;
Wood, TC ;
Mao, L ;
Quail, P ;
Wing, R ;
Dean, R ;
Yu, YS ;
Zharkikh, A ;
Shen, R ;
Sahasrabudhe, S ;
Thomas, A ;
Cannings, R ;
Gutin, A ;
Pruss, D ;
Reid, J ;
Tavtigian, S ;
Mitchell, J ;
Eldredge, G ;
Scholl, T ;
Miller, RM ;
Bhatnagar, S ;
Adey, N ;
Rubano, T ;
Tusneem, N .
SCIENCE, 2002, 296 (5565) :92-100
[7]   Relationship between ultraviolet-B sensitivity and cyclobutane pyrimidine dimer photorepair in rice [J].
Hidema, J ;
Song, IK ;
Sato, T ;
Kumagai, T .
JOURNAL OF RADIATION RESEARCH, 2001, 42 (03) :295-303
[8]   Ultraviolet B-sensitive rice cultivar deficient in cyclobutyl pyrimidine dimer repair [J].
Hidema, J ;
Kumagai, T ;
Sutherland, JC ;
Sutherland, BM .
PLANT PHYSIOLOGY, 1997, 113 (01) :39-44
[9]   Differences in the sensitivity to UVB radiation of two cultivars of rice (Oryza sativa L) [J].
Hidema, J ;
Kang, HS ;
Kumagai, T .
PLANT AND CELL PHYSIOLOGY, 1996, 37 (06) :742-747
[10]   UV radiation-sensitive Norin 1 rice contains defective cyclobutane pyrimidine dimer photolyase [J].
Hidema, J ;
Kumagai, T ;
Sutherland, BM .
PLANT CELL, 2000, 12 (09) :1569-1578